Quantitative Evaluation of Mucosal Mast Cells in the Colon in Patients with Irritable Bowel Syndrome

Authors

  • Md Abdul Wadud Registrar, (Gastroenterology), Khulna Medical College Hospital, Khulna, Bangladesh
  • Mohammad Mosiur Rahman Associate Professor, Department of Pathology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh
  • Muhammed Arshad Ul Azim Assistant Professor, Department of Nephrology, Shaheed Sheikh Abu Naser Specialized Hospital, Khulna, Bangladesh
  • Sheikh Abdullah Al Mamun Junior Consultant, Medicine, Shaheed Sheikh Abu Naser Specialized Hospital, Khulna, Bangladesh
  • Md Shafiqul Islam Assistant Professor, Department of Gastroenterology, Rajshahi Medical College, Rajshahi, Bangladesh
  • Ashfaque Ahmed Siddique Assistant Professor, Sheikh Russel National Gastroliver Institute and Hospital, Dhaka, Bangladesh
  • Md Rofiqul Islam Assistant Professor, Department of Hepatology, Rajshahi Medical College, Rajshahi, Bangladesh

DOI:

https://doi.org/10.3329/taj.v34i2.58550

Keywords:

Mast cell, Irritable bowel syndrome

Abstract

Background: Recent studies have shown that mast cells play an important role in the pathogenesis of irritable bowel syndrome as they release variable mediators which alter enteric nerve and smooth muscle function. The aim of this study was to determine whether mucosal mast cells were increased in the colonic mucosa of IBS patients compared to controls.

Materials and Methods: This case-control study was conducted in the Department of Gastroenterology, Bangabandhu Sheikh Mujib Medical University, Dhaka, during the period from April 2016 to March 2017 on cases of IBS and comparison group. We recruited 25 IBS patients and 25 healthy controls for this study. Colonoscopic biopsy was taken from the caecum, ascending colon, descending colon, and rectum. Tissue was stained with Giemsa, and then quantitative evaluation of mast cells was performed. Mast cells counts were compared between the two groups of patients.

Results: Mast cells were significantly higher in the caecum, ascending colon, descending colon, and rectum in all subtypes of IBS patients compared to control (10.40±2.10, 6.76±1.83, 8.08±2.19, and 9.16±2.46 vs. 4.20±1.01, 3.32±0.69, 3.04±0.84, and 3.84±1.07 per HPF, respectively). Among four sites, mast cells were significantly higher in the caecum in IBS patients. Mucosal mast cells were relatively elevated in IBS-D patients compared to IBS-C and IBS-M patients, but this was not statistically significant.

Conclusion: Mast cells were significantly increased in the caecum, ascending colon, descending colon, and rectum of the patients with IBS compared to controls. These findings suggest that mast cells may play an essential role in the pathogenesis of IBS.

TAJ 2021; 34: No-2: 09-14

Downloads

Download data is not yet available.
Abstract
26
PDF
24

Downloads

Published

2022-03-08

How to Cite

Wadud, M. A. ., Rahman, M. M. ., Ul Azim, M. A. ., Al Mamun, S. A. ., Islam, M. S. ., Siddique, A. A. ., & Islam, M. R. . (2022). Quantitative Evaluation of Mucosal Mast Cells in the Colon in Patients with Irritable Bowel Syndrome. TAJ: Journal of Teachers Association, 34(2), 9–14. https://doi.org/10.3329/taj.v34i2.58550

Issue

Section

Original Articles