Occurrence of Clostridium perfringens in layer flocks of selected districts in Bangladesh: molecular typing, antimicrobial susceptibility

Authors

  • Mohammad Arif Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  • Nigar Sultana Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  • Sk Shaheenur Islam Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  • Arunima Oarin Tresha Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  • Sayed Abdullah-Al-Mamun Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  • Md Ashiquen Nobi Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  • Mohammad Ferdousur Rahman Khan Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  • SM Lutful Kabir Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

DOI:

https://doi.org/10.3329/aajbb.v7i2.60995

Keywords:

Clostridium perfringens, necrotic enteritis (NE), layer flocks, antimicrobial resistant, low-biosecurity, Bangladesh

Abstract

Necrotic enteritis (NE) is one of the important enteric diseases in poultry caused by Clostridium perfringens which leads to considerable economic losses for poultry raisers. The present research was carried out to estimate prevalence, risk factors and to confirm molecular pattern, antimicrobial susceptibility status of Clostridium perfringens in layer flocks in Mymensingh and Gazipur districts of Bangladesh, where a total of 287 samples were collected that comprised of water (30), workers’ hand washing (30), and feed (30) as environmental pooled samples; cloacal swab (150) and post mortem sample (intestine) (47) as individual samples, and evaluated by basic (culture, biochemical test), and finally toxinotyping multiplex PCR assays. The flock-level data on potential risk factors were collected through semi-structured interviews with the farm owners and evaluated via univariable and multivariable logistic regression analyses where a p-value of <0.05 was considered as statistically significant. Overall flock-level prevalence of Clostridium perfringens was confirmed to be 6.3% (95% confidence interval [CI]: 3.8-9.7%). The occurrence of C. perfringens was found to be higher in the intestine samples (12.8%, 95% CI: 4.8- 25.7%) followed by feed (6.7%, 95% CI: 0.8-22.1%), cloacal swabs (6.0%, 95% CI: 2.8-11.1%) and workers’ hand washing (3.3%, 95% CI: 0.1- 12.2%). History of immune-suppressive disease (like New castle disease/egg drop syndrome/infectious bursal disease) [Odds Ratio (OR) = 22.16, 95% CI: 1.40-349.36, P = 0.027] and flock size more than 3000 birds (OR = 11.16, 95% CI: 1.26-98.25, P = 0.029) were found to be associated with C. perfringens infection status in the layer flocks. In this study, C. perfringens toxinotype A is circulating in layer flocks with 72.2% of isolates were established as multidrug-resistant as they demonstrated resistance between 3 to 6 antimicrobial agents. Evidence-based control measures to be taken namely use of probiotics, prebiotic, organic acids, and plant extracts as natural alternatives including control of immune-suppressive diseases are needed to minimize the NE infection and antimicrobial resistance in low biosecurity layer flocks.

Asian Australas. J. Biosci. Biotechnol. 2022, 7(2), 36-49

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Published

2022-08-16

How to Cite

Arif, M., Sultana, N. ., Islam, S. S. ., Tresha, A. O. ., Abdullah-Al-Mamun, S., Nobi, M. A. ., Khan, M. F. R. ., & Kabir, S. L. . (2022). Occurrence of Clostridium perfringens in layer flocks of selected districts in Bangladesh: molecular typing, antimicrobial susceptibility. Asian-Australasian Journal of Bioscience and Biotechnology, 7(2), 36–49. https://doi.org/10.3329/aajbb.v7i2.60995

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Section

Research Articles