Effect of growth regulators on in vitro multiplication of potato (Solanum tuberosum L.) cv. diamant

Authors

  • Md Shoyeb Department of Genetic Engineering and Biotechnology, Faculty of Biological Science, Jessore University of Science and Technology, Jessore-7408, Bangladesh
  • Md Saddam Hossain Department of Genetic Engineering and Biotechnology, Faculty of Biological Science, Jessore University of Science and Technology, Jessore-7408, Bangladesh
  • Md Saiful Islam Department of Genetic Engineering and Biotechnology, Faculty of Biological Science, Jessore University of Science and Technology, Jessore-7408, Bangladesh
  • Afifa Azad Department of Genetic Engineering and Biotechnology, Faculty of Biological Science, Jessore University of Science and Technology, Jessore-7408, Bangladesh
  • Md Abdur Rauf Sarkar Department of Genetic Engineering and Biotechnology, Faculty of Biological Science, Jessore University of Science and Technology, Jessore-7408, Bangladesh
  • Shaikh Mizanur Rahman Department of Genetic Engineering and Biotechnology, Faculty of Biological Science, Jessore University of Science and Technology, Jessore-7408, Bangladesh

DOI:

https://doi.org/10.3329/aajbb.v2i1.64350

Keywords:

potato, shoot tips, micropropagation, BAP, GA3

Abstract

Shoot tip and nodal segment explants from field grown plants were used as experimental materials in this investigation. All explants were cultured on MS medium supplemented with various plant growth regulators. For surface sterilization of explants, HgCl2 (0.1%) for 2 minutes was found to be most effective for complete killing of surface pathogens and getting healthy tissues. Shoot regeneration was observed from both shoot tips and nodal explants for the studied plant. Various concentrations of BAP (0.1, 0.2, 0.3mg/l) and GA3 (0.1, 0.2, 0.3mg/l) were used for shoot multiplication. In case of BAP, the highest length of shoot was recorded 4 cm and the highest percentage of shoot multiplication (73%) was noticed in MS+0.2mg/l BAP. And in case of GA3, the highest response for shoot multiplication (82%) was noticed in MS+ 0.1 mg/l GA3.But among all of the media formulations used in this experiment, the highest response for shoot multiplication (95%) within7-10 days was noticed in hormone free MS media. In case of root regeneration, the highest percentage (96%) of root induction was recorded in MS medium supplemented with no hormone.

Asian Australas. J. Biosci. Biotechnol. 2017, 2 (1), 81-88

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Published

2017-04-30

How to Cite

Shoyeb, M., Hossain, M. S. ., Islam, M. S. ., Azad, A., Sarkar, M. A. R. ., & Rahman, S. M. . (2017). Effect of growth regulators on in vitro multiplication of potato (Solanum tuberosum L.) cv. diamant. Asian-Australasian Journal of Bioscience and Biotechnology, 2(1), 81–88. https://doi.org/10.3329/aajbb.v2i1.64350

Issue

Vol. 2 No. 1 (2017)

Section

Research Articles

License

Copyright (c) 2017 Md Shoyeb, Md Saddam Hossain, Md Saiful Islam, Afifa Azad, Md Abdur Rauf Sarkar, Shaikh Mizanur Rahman

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.