Cardioprotective effects of native herb-derived cardiac tonic on infarct size in a mouse model of experimental myocardial infarction
DOI:
https://doi.org/10.3329/aajbb.v9i2.73691Keywords:
herbal extract, coronary infarction, mortality, animal model, remodelingAbstract
Cardiac remodeling is a significant issue involving molecular, cellular, and interstitial changes that affect the size, mass, integrity, and function of the heart following a myocardial infarction (MI). We hypothesized that therapy with mixed herbal extracts has a protective effect in mouse models of MI. The underlying mechanisms are thought to be protective against left ventricular (LV) remodeling through reduced collagen deposition. We therefore studied the effects of mixed herbal extracts commonly used in various ways in the belief that they have beneficial effects on cardiac disease. MI was induced by left anterior descending (LAD) coronary artery ligation in switch albino mice. Ethanol extracts of herbs Arjuna bark, oleander leaves, ginseng root, garlic, cardamom seeds and oregano leaves were prepared by ethanol extraction and rotary evaporation technique of dried herbs and then mixed the extracts to make a therapeutic cocktail. The mixture is then administered orally by lavage for 28 days. At the end of 28 days cardiac tissues were harvested and stored for histopathology analysis and assessment of SOD levels. Survival curve data over a period of 28 days showed significant (P<0.05) differences between groups and mortality was reduced in the herbal extract treated group. Heart mass especially ventricular and atrial space and ratio of infarct to total heart area revealed better improvement in treated group compared to untreated group. Histopathological studies showed that the myocardium of the herbal extract treated group had fewer collagen fibers than the non-treated group. SOD activity levels were up-regulated in animals treated with herbal extracts indicating reduction of oxidative stress by herbal extract mixture. Hence, their effects reduce collagen deposition, reduce LV mass and prevent myocardial tissue damage, reduce structural changes and increase survival by their anti-stress effects. Mixed herbal tonic may provide a new therapeutic approach in ischemic cardiomyopathy and ischemic heart failure.
Asian Australas. J. Biosci. Biotechnol. 2024, 9(2), 14-23
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Abdou RH, WA Basha and WF Khalil, 2019. Subacute toxicity of Nerium oleander ethanolic extract in mice. Toxicol. Res., 35: 233-239.
Adome RO, JW Gachihi, B Onegi, J Tamale and SO Apio, 2003. The cardiotonic effect of the crude ethanolic extract of Nerium oleander in the isolated guinea pig hearts. African Heal. Sci., 3: 77-82.
Ashraf R, RA Khan, I Ashraf and AA Qureshi, 2013. Effects of Allium sativum (garlic) on systolic and diastolic blood pressure in patients with essential hypertension. Pak. J. Pharm. Sci., 26: 859-863.
Amalraj A and S Gopi, 2016. Medicinal properties of Terminalia arjuna (Roxb.) Wight & Arn.: A review. J. Tradit. Complement. Med., 7: 65-78.
Aslani MR, AR Movassaghi, M Mohri, A Abbasian and M Zarehpour, 2004. Clinical and pathological aspect of experiment oleander (Nerium oleander) toxicosis in sheep. Vet. Res. Commun., 28: 609-616.
Brent AF and MK Christopher, 2007. Mechanisms of post-infarct left ventricular remodeling. Drug Discov. Today Dis. Mech., 4: 185-196.
Cheng C, Y Zou and J Peng, 2018. Oregano essential oil attenuates RAW264.7 cells from lipopolysaccharide-induced inflammatory response through regulating NADPH oxidase activation-driven oxidative stress. Molecules, 23: 1857.
Dabravolski SA, VN Sukhorukov, AA Melnichenko, VA Khotina and AN Orekhov, 2023. Potential application of the plant-derived essential oils for atherosclerosis treatment: Molecular mechanisms and therapeutic potential. Molecules, 28: 5673.
Dwivedi S and D Chopra, 2014. Revisiting Terminalia arjuna - An ancient cardiovascular drug. J. Tradit. Complement. Med., 4: 224-231.
Dwivedi S, A Aggarwal, MP Agarwal and S Rajpal, 2005. Role of Terminalia arjuna in ischaemic mitral regurgitation. Int. J. Cardiol., 100: 507-508.
Galey FD, DM Holstege, KH Pulmee, E Tor, B Johnson, ML Anderson, PC Blanchard and F Brown, 1996. Diagnosis of oleander poisoning in livestock. J. Vet. Diagn. Investig., 8: 358-364.
Gayathri V, S Ananthi, C Chandronitha, G Ramakrishnan, R Lakshmisundaram, RL Sundaram and HR Vasanthi, 2011. Cardioprotective effect of Nerium oleander flower against isoproterenol-induced myocardial oxidative stress in experimental rats. J. Cardiovasc. Pharmacol. Ther., 16: 96-104.
Goyal SN, C Sharma, UB Mahajan, CR Patil, YO Agrawal, S Kumari, DS Arya and S Ojha, 2015. Protective effects of cardamom in isoproterenol-induced myocardial infarction in rats. Int. J. Mol. Sci., 16: 27457-27469.
Harvey A, 2000. Strategies for discovering drugs from previously unexplored natural products. Drug Disc. Today, 7: 294-300.
Kim ND, SY Kang, MJ Kim, JH Park and VB Schini-Kerth, 1999. The ginsenoside Rg3 evokes endothelium-independent relaxation in rat aortic rings: role of K+ channels. Eur. J. Pharmacol., 367: 51-57.
Laslett LJ, P Alagona, BA Clark 3rd, JP Drozda Jr, F Saldivar, SR Wilson, C Poe and M Hart, 2012. The worldwide environment of cardiovascular disease: prevalence, diagnosis, therapy, and policy issues: a report from the American college of cardiology. J. Am. Coll. Cardiol., 60: S1-S49.
Lee DC, MO Lee, CY Kim and DH Clifford, 1981. Effect of ether, ethanol and aqueous extracts of ginseng on cardiovascular function in dogs. Can. J. Comp. Med., 45: 182-187.
Mann DL, 2015. Innate immunity and the failing heart: the cytokine hypothesis revisited. Circ. Res., 116: 1254-1268.
Maruyama K, K Naemura, K Yoshihara, K Imanaka-Yoshida, H Kurihara and S Miyagawa-Tomita, 2021. Surgical protocol for permanent ligation of the left anterior descending coronary artery in mice to generate a model of myocardial infarction. STAR Protoc., 26: 100775.
Maulik SK and S Kumar, 2012. Oxidative stress and cardiac hypertrophy: a review. Toxicol. Mech. Methods, 22: 359-366.
Moe GW and J Marín-García, 2016. Role of cell death in the progression of heart failure. Heart Fail. Rev., 21: 157-167.
Mwafy SN and MM Yassin, 2011. Antidiabetic activity evaluation of glimepiride and Nerium oleander extract on insulin, glucose levels and some liver enzymes activities in experimental diabetic rat model. Pak. J. Biol. Sci., 14: 984-990.
Prabhu SD and NG Frangogiannis, 2016. The biological basis for cardiac repair after myocardial infarction: From inflammation to fibrosis. Circ. Res., 24: 91-112.
Rahman K and B David, 2000. Dietary supplementation with aged garlic extract inhibits adp-induced platelet aggregation in humans. J. Nutri., 130: 2662-2665.
Rosamond W, K Flegal, G Friday, K Furie, A Go, K Greenlund, N Haase, M Ho, V Howard, B Kissela, S Kittner, D Lloyd-Jones, M McDermott, J Meigs, C Moy, G Nichol, CJ O'Donnell, V Roger, J Rumsfeld, P Sorlie, J Steinberger, T Thom, S Wasserthiel-Smoller and Y Hong, 2007. Heart disease and stroke statistics--2007 update: a report from the American Heart Association Statistics Committee and Stroke Statistics Subcommittee. Circulation, 6: e69-171.
Shridhar NB, 2022. Nerium oleander toxicity: A review. Int. J. Adv. Aca. Stud., 4: 23-32.
Subramaniam S, R Subramaniam, S Rajapandian, S Uthrapathi, VR Gnanamanickam and GP Dubey, 2011. Anti-atherogenic activity of ethanolic fraction of Terminalia arjuna bark on hypercholesterolemic rabbits. Evid. Based Complement. Alternat. Med., 2011: 487916.
Sun YE, W Wang and J Qin, 2018. Anti-hyperlipidemia of garlic by reducing the level of total cholesterol and low-density lipoprotein: A meta-analysis. Medicine, 97: e0255.
Tang MM, ST Zhao, RQ Li and W Hou, 2023. Therapeutic mechanisms of ginseng in coronary heart disease. Front. Pharmacol., 3: 1271029.
Teringova E and P Tousek, 2017. Apoptosis in ischemic heart disease. J. Transl. Med., 15: 87.
Tsai TH, LT Chuang, TJ Lien, YR Liing, WY Chen and PJ Tsai, 2013. Rosmarinus officinalis extract suppresses Propionibacterium acnes-induced inflammatory responses. J. Med. Food., 16: 324-333.
Verma S, A Bordia and V Jain, 2010. Greater cardamom (Amomum subulatum Roxb.) –A cardio-adaptogen against physical stress. J. Herb. Med. Toxicol., 4: 55-58.
Zannad F, P Rossignol and W Iraqi, 2010. Extracellular matrix fibrotic markers in heart failure. Heart Fail. Rev., 15: 319-329.
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Copyright (c) 2024 Md Mizanur Rahman, Md Shahidul Islam, Md Tasmir Rayan Labib, Md Sodrul Islam, Kazi Khalid Ibne Khalil, Apurbo Kumar Mondal, Md Abdullah Al Mahmud
This work is licensed under a Creative Commons Attribution 4.0 International License.
