Development of <i>Lloplasmic brassica</i> species: variation in DNA contents and its relationshop with morphlogical traits in C2 generation

Abstract

Five C2 populations and their parents were studied for subsequent development of alloplasmic species using appropriate methods. The study was aimed at finding out the variation in morphological traits, DNA content and their relationship at C2 generation with respect to their parents. DNA contents of the cross products of BINA-4 à Agrani, BINA-4 à Safal, BINA-4 à Daulat and BINA-5 à Daulat were 480, 620, 610, and 720 ng/µl, respectively. In all the cases of B. napocampestris and B. napojuncea materials, the DNA contents were much higher than their parental values. The DNA content of BINA-4 à Agrani (B.napus x B.rapa) and BINA-4 à Daulat (B.npus x B.juncea) were significantly correlated in case of pollen fertility and leaf area, while the correlated response of the crosses BINA-5 à Sampad (B.napus à B.rapa) and BINA-5 à Daulat (B.napus à B.juncea) were significant in case of leaf area only. The cross BINA-5 à Daulat showed strong correlation with DNA content in case of plant height, BINA-4 à Daulat and BINA-4 à Safal in case of beak length, while BINA-4 à Safal in case of seeds per siliqua only. The results indicate that the changes of DNA content also cause changes in morphological traits of the cross products of two different species having different genomic combinations.

Bangladesh J. Agril. Res. 32(4) 509-517