Genetic diversity of fruit borer, Helicoverpa armigera (Lepidoptera: Noctuidae) based on random amplified polymorphic DNA- polymerase chain reaction

Authors

  • AKMZ Rahman Senior Scientific Officer and Chief Scientific Officer, respectively, Entomology Division, Bangladesh Agricultural Research Institute (BARI), Gazipur-1701
  • MA Haque Professor, Department of Entomology, Bangladesh Agricultural University (BAU), Mymensingh
  • SN Alam Senior Scientific Officer and Chief Scientific Officer, respectively, Entomology Division, Bangladesh Agricultural Research Institute (BARI), Gazipur-1701
  • P Yasodha Assistant Professor (Agril. Entomology), Department of Agril. Processing & Basic Sciences, Agricultural Engineering College and Research Institute, Kumulur, Trichi, Tamil Nadu
  • V Balasubramani Professor (Agril, Entomolology), Dept. of Plant Biotechnology, CPMB, Tamil Nadu Agricultural University, Tamil Nadu

DOI:

https://doi.org/10.3329/bjar.v39i2.20428

Keywords:

Helicoverpa armigera, genetic diversity, RAPD-PCR, primers

Abstract

The genetic variability of Helicoverpa armigera (Hübner) at different agroecological zones of Bangladesh in comparison with Indian population was conducted in India during September 2008 to February 2009. A total of 12 H. armigera populations of which 10 populations collected from different agroecological zones of Bangladesh and two populations from India were tested for their genetic variability. Eight out of the ten primers produced scorable PCR products by amplifying the template DNA with taq polymerase and were subjected for analysis. Those eight primers got amplified to a total of 138 markers which produced polymorphic markers. The similarity coefficient based on 138 RAPD markers ranged from 0.000 to 0.777 of the pair-wise combination among twelve samples of H. armigera. An UPGMA dendrogram based on Jaccards similarity coefficient was constructed for the 12 samples of H. armigera. The dendrogram showed that H. armigera population from Bangladesh had 25 to 45 percent similarity, and in its Indian population the similarity remained within this range.

DOI: http://dx.doi.org/10.3329/bjar.v39i2.20428

Bangladesh J. Agril. Res. 39(2): 263-271, June 2014

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Published

2014-09-12

How to Cite

Rahman, A., Haque, M., Alam, S., Yasodha, P., & Balasubramani, V. (2014). Genetic diversity of fruit borer, Helicoverpa armigera (Lepidoptera: Noctuidae) based on random amplified polymorphic DNA- polymerase chain reaction. Bangladesh Journal of Agricultural Research, 39(2), 263–271. https://doi.org/10.3329/bjar.v39i2.20428

Issue

Vol. 39 No. 2 (2014)

Section

Articles