In vitro root formation and plantlet development in dendrobium orchid

Authors

  • MM Khatun Biotechnology Division, Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur
  • H Khatun Department of Biotechnology, Bangladesh Agricultural University (BAU), Mymensingh
  • D Khanam Biotechnology Division, Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur
  • Md Al-Amin Biotechnology Division, Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur

Keywords:

Orchid, Dendrobium, hybrid, In vitro rooting

Abstract

The experiment was conducted to investigate the combined effect of different plant growth regulators with and without charcoal supplementation for root formation and plantlet development from protocorm like bodies (PLBS) of orchid. The combination of BAP + NAA, BAP + IAA, BAP + IBA, and IAA + IBA at different concentrations were studied. It revealed that the highest number of roots was obtained from 1.0 mg/L each of IAA + IBA combination (6.667) and the highest root length was recorded from 2.0 mg/L BAP + 1.0 mg/L IBA with charcoal supplementation. The treatment combinations, 1.0 mg/L each of BAP + NAA, BAP + IAA, BAP + IBA, and IAA + IBA were found best for producing more rooted plantlets with charcoal supplementation. It revealed that charcoal enhanced the root formation.

Keywords: Orchid; Dendrobium; hybrid; In vitro rooting.

DOI: 10.3329/bjar.v35i2.5888

Bangladesh J. Agril. Res. 35(2) : 257-265, June 2010
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How to Cite

In vitro root formation and plantlet development in dendrobium orchid. (2010). Bangladesh Journal of Agricultural Research, 35(2), 257-265. https://doi.org/10.3329/bjar.v35i2.5888

Issue

Vol. 35 No. 2 (2010)

Section

Articles

How to Cite

In vitro root formation and plantlet development in dendrobium orchid. (2010). Bangladesh Journal of Agricultural Research, 35(2), 257-265. https://doi.org/10.3329/bjar.v35i2.5888