Molecular characterization of hyacinth bean germplasm collected from Bangladesh and exotic sources

Abstract

Hyacinth bean (Lablab purpureus (L.) Sweet) has versatile use as vegetable, pulse, fodder, hay, silage, green manure and cover crop. The experiment was conducted with 65 accessions of hyacinth bean from 17 countries of Asia, Africa and Europe for molecular characterization and phylogenetic relationship. Simple sequence repeat marker (SSR) derived from Medicago and cowpea was used. A total of 47 polymorphic alleles were  counted. Two to five alleles per marker were detected with an average of 3.62. PIC values ranged from 0.21 to 0.72 with a mean of 0.53. Average gene diversity was exhibited 0.59 ranging from of 0.24 to 0.76. The maximum genetic distance (0.86) was observed between accessions of Bangladesh and India. Phylogenetic analysis classified the 65 accessions into 8 groups ranging from 1 to 22 accessions per group. Small values of genetic dissimilarities (0 to 0.29) showed without relating geographic distribution. Comparatively greater diversity was exhibited among the accessions in Bangladesh than the remaining 16 countries. The analysis of molecular variance indicated a fixation index of 0.0399 suggesting a low differentiation among the hyacinth bean accessions in Bangladesh and free gene flow among the populations. Genetically diverse accessions were identified that could be potentially source of germplasm for further crop improvement in the country.

Bangladesh J. Agril. Res. 48(2): 137-160, June 2023