In vitro maturation of buffalo oocytes and fertilization by cattle spermatozoa

Authors

  • MAMY Khandoker Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh 2202
  • MMT Reza Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh 2202
  • LY Asad Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh 2202
  • S Saha Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh 2202
  • AS Apu Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh 2202
  • SAM Hoque Department of Animal Breeding and Genetics, Bangabandu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706

DOI:

https://doi.org/10.3329/bjas.v41i1.11969

Keywords:

In vitro maturation, in vitro fertilization, buffalo oocytes, cattle spermatozoa

Abstract

The present research was undertaken to explore the maturation of buffalo oocytes using bovine follicular fluid (BFF) and bovine serum albumin (BSA), as well as subsequent fertilization using cattle spermatozoa. The cumulus oocyte complexes (COCs) were collected by aspiration of slaughterhouse buffalo ovaries. Maturation was performed in TCM 199 supplemented with 10% BFF, 5% BSA or without supplementation (control). The COCs were fertilized in Brackett and Oliphant (BO) medium using capacitated fresh cattle spermatozoa. It was observed that the percentage of COCs reached to M-II stages were 40.78±3.84, 65.74±2.39 and 67.52±0.85; normal fertilization (formation of 2 pronuclei) were 23.28±3.00, 29.30±0.73 and 30.52±1.21 for control, 10% BFF and 5% BSA supplementation, respectively. The supplementation of BFF (10%) and BSA (5%) were given similar results on maturation and increased significantly (p<0.05) than that of the control. It was observed that cattle spermatozoa were fertilized by the buffalo oocytes and the fertilization rate was 23.28% to 30.52% in BFA and BSA supplemented media, respectively. It can be concluded that buffalo oocytes might be fertilized using capacitated cattle spermatozoa and both 10% BFF and 5% BSA could be supplemented in maturation media to enhance the maturation rate as well as fertilization of buffalo oocytes.

http://dx.doi.org/10.3329/bjas.v41i1.11969

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Published

2012-06-01

How to Cite

Khandoker, M., Reza, M., Asad, L., Saha, S., Apu, A., & Hoque, S. (2012). In vitro maturation of buffalo oocytes and fertilization by cattle spermatozoa. Bangladesh Journal of Animal Science, 41(1), 6–12. https://doi.org/10.3329/bjas.v41i1.11969

Issue

Vol. 41 No. 1 (2012)

Section

Articles

License

© Bangladesh Journal of Animal Science.

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