Thermally induced aggregation and its suppression of silkworm small heat shock protein sHSP19.9

Authors

  • MT Hossain Department of Biochemistry and Molecular Biology, Bangladesh Agricultural University, Mymensingh-2202
  • Y Aso Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581

DOI:

https://doi.org/10.3329/bjas.v46i1.32179

Keywords:

sHSP19.9, thermal aggregation, silkworm, suppression of aggregation

Abstract

About ten genes responsible for small heat-shock proteins (sHSP) have been isolated from silkworm. sHSP19.9 is one of the important member among them. Heat-induced stability of the sHSP was investigated at 60ºC with 20 mM HEPES buffer pH 7.7 containing 10 mM NaCl (low-ionic strength). Along with it probable suppression of the aggregation was also examined. At the mentioned reaction medium, sHSP19.9 was observed to be aggregated on the concentration- and time-dependent manners. It was successfully suppressed with dithiothreitol (DTT), higher-ionic strengths, Cysteine residues modifications and molecular chaperone: sHSP20.8.

Bang. J. Anim. Sci. 2017. 46 (1): 57-64

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Published

2017-04-13

How to Cite

Hossain, M., & Aso, Y. (2017). Thermally induced aggregation and its suppression of silkworm small heat shock protein sHSP19.9. Bangladesh Journal of Animal Science, 46(1), 57–64. https://doi.org/10.3329/bjas.v46i1.32179

Issue

Vol. 46 No. 1 (2017)

Section

Articles

License

© Bangladesh Journal of Animal Science.

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