In-silico and in-vitro Antibacterial Activity and GC-MS Analysis of Anogeissus acuminata Leaf Extract with Host Toxicity Testing

Authors

  • J Bikrant Kumar Prusty Department of Pediatrics, Institute of Medical Sciences and SUM Hospital, Siksha ‘O’ Anusandhan deemed to be University, K8, Kalinga Nagar, Bhubaneswar-751003, Odisha, India. https://orcid.org/0000-0002-9326-7163
  • Monali Priyadarsini Mishra Central lab, School of Paramedics and Allied Health Sciences, Centurion University of Technology and Management, Jatni-752050, Bhubaneswar, Odisha, India. https://orcid.org/0000-0002-8548-1784
  • Rabindra Nath Padhy Central Research Laboratory, IMS and Sum Hospital, Siksha ‘O’ Anusandhan University, K-8, Kalinga Nagar, Bhubaneswar-751003, Odisha, India https://orcid.org/0000-0002-2522-9843

DOI:

https://doi.org/10.3329/bjid.v11i2.70651

Keywords:

Anogeissus Acuminata, GC-MS Analysis, Molecular Docking, Cytotoxicity

Abstract

Background: Anogeissus acuminata leaf extract is utilized in the different purposes.

Objective: Antibacterial activity of organic solvent fractions of crude leaf extract of Anogeissus acuminata was used against multidrug-resistant strains of 9 pathogenic bacteria isolated from urine samples of patients with urinary tract infections.

Methodology: The n-butanol fraction revealed effective control over pathogens, and the zone size was 21 mm as a measure of antibacterial activity against Proteus mirabilis. The gas chromatography-mass spectroscopy analysis indicated the presence of 15 phyto-compounds with docking score values -5 to -7 kcal/mol in a molecular docking attempt against β-lactamase of Pseudomonas aeruginosa.

Results: The isolated chemicals, conocarpan and dihydrodehydrodiconiferyl alcohol, had comparatively effective docking scores, -10.908 and -10.081 kcal/mol, respectively. In toxicity testing, the experimental minimum inhibitory concentration value was 600 mg/L, and the computed lethal concentration25 value was 1698.24 mg/L during cytotoxicity assay; it was observed that comet was not found in the cells treated leaf extract.

Conclusion: The isolated compounds conocarpan and dihydrodehydrodiconiferyl alcohol achieved successful docking scores against -lactamase in the molecular docking attempt. When the leaf extract was seen using lymphocytes grown from UCB, it was determined that it had no host toxicity.

Bangladesh Journal of Infectious Diseases, December 2024;11(2):93-101

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Published

2025-02-18

How to Cite

Prusty, J. B. K., Mishra, M. P., & Padhy, R. N. (2025). In-silico and in-vitro Antibacterial Activity and GC-MS Analysis of Anogeissus acuminata Leaf Extract with Host Toxicity Testing. Bangladesh Journal of Infectious Diseases, 11(2), 93–101. https://doi.org/10.3329/bjid.v11i2.70651

Issue

Vol. 11 No. 2 (2024)

Section

Original Articles

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Copyright (c) 2024 J Bikrant Kumar Prusty, Monali Priyadarsini Mishra, Sashank Sekhar Swain, Rabindra Nath Padhy

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