Establishment of Efficient Protocols for In Vitro Regeneration of Hyptis Suaveolens (L.) Poit. Using Apical Shoots and Nodal Segments

Authors

  • M A Kashem Department of Botany, University of Chittagong, Chittagong - 4331, Bangladesh
  • M M Rahman Department of Botany, University of Chittagong, Chittagong - 4331, Bangladesh

DOI:

https://doi.org/10.3329/cujbs.v8i1.73530

Keywords:

Hyptis suaveolens, Apical Shoot and Nodal segments, in vitro regeneration, aromatic medicinal plant

Abstract

The present work was undertaken with a view to standardize an efficient protocol for in vitro rapid propagation of Hyptis suaveolens. Shoot apices and nodal segments of H. suaveolens produced multiple shoot buds when cultured on MS medium supplemented with 1.5-3.0 mg/l BAP or Kn in the combinations with 0.5-1.0 mg/l IAA or NAA. Nodal explants exhibited highest proliferation (90%) and gave maximum number of shoots per culture (8.40 ± 0.18) on MS medium fortified with 1.5 mg/I BAP and 1.0 mg/l IAA. The shoot buds were elongated on MS medium fortified with different concentrations and combinations of auxins and cytokinins. Elongated shoot buds were further cultured in rooting media for root induction. One fourth MS medium -without PGR was - proved ·the most effective for - induction and proliferation of roots. The--rooted plantlets ·were 'established in the field through successive phases of acclimation and the survival rate was 70%.

The Chittagong Univ. J B. Sci. 8(1&2): 99-105, 2013

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Published

2024-05-28

How to Cite

Kashem, M. A., & Rahman, M. M. (2024). Establishment of Efficient Protocols for In Vitro Regeneration of Hyptis Suaveolens (L.) Poit. Using Apical Shoots and Nodal Segments. Chittagong University Journal of Biological Sciences, 8(1), 99–105. https://doi.org/10.3329/cujbs.v8i1.73530

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