Development and Validation of RP-HPLC method for determination of Modafinil in bulk and dosage form(3ò¬

Authors

  • Chusena Narasimha Raju Bhimanadhuni Research Scholar, Department of Pharmaceutical analysis, Prist University, Thanjavur, Tamilnadu
  • Devala Rao Garikapati Department of Pharmaceutical analysis, K.V.S.R Siddhartha College of pharmaceutical sciences, Vijayawada, Krishna(Dt), Andhra Pradesh
  • Swetha Karamsetty Department of Pharmaceutical analysis, Browns college of Pharmacy, Khammam, Andhra Pradesh

DOI:

https://doi.org/10.3329/icpj.v1i4.10062

Keywords:

Method development, validation, Modafinil, Tablets, Hypersil C18 Column, RP-HPLC

Abstract

A reverse phase high performance liquid chromatographic method was developed for the determination of Modafinil in bulk and dosage form. The separation was effective on a Hypersil ODS C18 column (250 mm x 4.6 mm; 5μ) using a mobile phase mixture of Buffer:Acetonitrile in a ratio of 55:45 (v/v) at a flow rate of 1.0ml/min. The detection was made at 220nm. The retention time of modafinil was found to be 4.80±0.06 min. Calibration curve was linear over the concentration range of 20-120μg/ml of modafinil. The proposed method was validated as per the ICH guidelines. The method was accurate, precise, specific and rapid and thus found to be suitable for the quantitative analysis of modafi-nil in the bulk and dosage form.

Key Words: Method development; validation; Modafinil; Tablets; Hypersil C18 Column; RP-HPLC.

DOI: http://dx.doi.org/10.3329/icpj.v1i4.10062

International Current Pharmaceutical Journal 2012, 1(4): 77-80

 

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How to Cite

Bhimanadhuni, C. N. R., Garikapati, D. R., & Karamsetty, S. (2012). Development and Validation of RP-HPLC method for determination of Modafinil in bulk and dosage form(3ò¬. International Current Pharmaceutical Journal, 1(4), 77–80. https://doi.org/10.3329/icpj.v1i4.10062

Issue

Vol. 1 No. 4 (2012)

Section

Original Articles

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