Development and validation of RP-HPLC method for determination of Duloxetine hydrochloride in bulk and dosage form

Authors

  • Chusena Narasimharaju Bhimanadhuni Research Scholar, Department of Pharmaceutical Analysis, Prist University, Thanjavur, Tamilnadu
  • Devala Rao Garikapati Department of Pharmaceutical Analysis, K.V.S.R Siddhartha College of Pharmaceutical Sciences, Vijayawada, Krishna(Dt), Andhra Pradesh
  • Chintha Srinivas Department of Pharmaceutical Analysis, Browns College of Pharmacy, Khammam, Andhra Pradesh

DOI:

https://doi.org/10.3329/icpj.v1i5.10281

Keywords:

Method development and validation, duloxetine hydrochloride, Tablets, Kromasil C18 column, RP-HPLC

Abstract

A reverse phase high performance liquid chromatographic method was developed for the determination of duloxetine hydrochloride in bulk and dosage form. The separation was effected on a kromasil ODS C18 column (250mmX4.6mm, 5?) using a mobile phase mixture of buffer and methanol in a ratio of 85:15 v/v at a flow rate of 1.0ml/min. The detection was made at 230nm. The retention time of duloxetine hydrochloride was found to be 3.443±0.06 min. Calibration curve was linear over the concentration range of 20-120?g/ml of duloxetine hydrochloride. The propose method was validated as per the ICH guidelines. The method was accurate, precise, specific and rapid found to be suitable for the quantitative analysis of the drug and dosage form.

DOI: http://dx.doi.org/10.3329/icpj.v1i5.10281

International Current Pharmaceutical Journal 2012, 1(5): 98-102

Downloads

Download data is not yet available.
Abstract
134
PDF
95

Downloads

Published

2012-04-07

How to Cite

Bhimanadhuni, C. N., Garikapati, D. R., & Srinivas, C. (2012). Development and validation of RP-HPLC method for determination of Duloxetine hydrochloride in bulk and dosage form. International Current Pharmaceutical Journal, 1(5), 98–102. https://doi.org/10.3329/icpj.v1i5.10281

Issue

Vol. 1 No. 5 (2012)

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

  1. The journal holds copyright and publishes the work under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).