PCR based detection of gyrB2 gene from Pseudomonas sp. affected human clinical isolates

Authors

  • Md. Javed Foysal Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet-3114
  • Gokul Chandra Biswas Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet-3114
  • Md. Jahir Raihan Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet-3114

DOI:

https://doi.org/10.3329/icpj.v1i9.11612

Keywords:

Pseudomonas sp., virulence gene, gyrB2, PCR detection, visualization

Abstract

Detection of virulence gene is a key component in determining the pathogenicity of any isolates because these genes act multi-functionally and multi-factorially. Gyrase specific gene primer in combination of PCR technology allows precise detection of DNA gyrase subunit B2 gene (gyrB2) from different virulent microorganisms. In the present study, forward and reverse primers with a length of 20bp for both were used for detection of gyrB2 genes in clinical isolates of Pseudomonas sp. collected from patients suffering from urinary tract infection (UTI). A total of 12 isolates of Pseudomonas sp. viz., Ps1, Ps2, Ps3, Ps4, Ps5, Ps6, Ps7, Ps8, Ps9, Ps10, Ps11 and Ps12 were used in present study in which gyrB2 gene amplified in all 12 isolates and gave the expected 1130bp PCR product after visualization under gel documentation system in 1.2% agarose gel. This PCR was outstanding in the detection of gyrB2 gene in urinary tract infected patients caused by Pseudomonas sp. species.

DOI: http://dx.doi.org/10.3329/icpj.v1i9.11612

International Current Pharmaceutical Journal 2012, 1(9): 235-238

 

Downloads

Download data is not yet available.
Abstract
104
PDF
60

Downloads

Published

2012-08-04

How to Cite

Foysal, M. J., Biswas, G. C., & Raihan, M. J. (2012). PCR based detection of gyrB2 gene from Pseudomonas sp. affected human clinical isolates. International Current Pharmaceutical Journal, 1(9), 235–238. https://doi.org/10.3329/icpj.v1i9.11612

Issue

Vol. 1 No. 9 (2012)

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

  1. The journal holds copyright and publishes the work under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).