Investigation of cytotoxicity and in-vitro antioxidant activity of Asparagus racemosus root extract

Authors

  • Md. Ismail Hossain Department of Pharmacy, University of Asia Pacific, Dhanmondi, Dhaka
  • Farzana Anwar Sharmin Department of Pharmacy, University of Asia Pacific, Dhanmondi, Dhaka
  • Sadika Akhter Department of Pharmacy, University of Asia Pacific, Dhanmondi, Dhaka
  • Mohiuddin Ahmed Bhuiyan Department of Pharmacy, University of Asia Pacific, Dhanmondi, Dhaka
  • Mohammad Shahriar Department of Pharmacy, University of Asia Pacific, Dhanmondi, Dhaka

DOI:

https://doi.org/10.3329/icpj.v1i9.11615

Keywords:

Radical scavenging, DPPH, phenolic, flavonoid, cupric, reducing power

Abstract

This attempt is made to address the phytoconstituents, free radical scavenging activity and brine shrimp lethality bioassay of five different extracts of Asparagus racemosus roots. Preliminary phytochemical examination of the crude extracts of Asparagus racemosus root disclosed the existence of different sort of chemical groups such as flavonoids, tannin, saponin, alkaloids, carbohydrate. The root displayed significant DPPH free radical scavenging activity with highest IC50 value showed by ethanol extract with a value of 78.15 µg/ml followed by methanol and petroleum ether having value of 106.44 µg/ml and 273.31 µg/ml respectively as opposed to that of the scavenging effects of ascorbic acid and BHT of 5.698 µg/ml and 8.816 µg/ml respectively. The highest reducing power was showed by ethanol extract followed by methanol and petroleum ether as opposed to that of the reducing potential of ascorbic acid and BHT. The fractions represented good cupric reducing capacity with increasing concentration taking ethanol extract in the top position. The ethanol extract yielded 108.78±2.77 mg/gm gallic acid equivalent phenolic content and methanol sub-fraction yielded 164.77±1.73 mg/gm quercetin equivalent flavonoid content that was highest among five extracts. Ethanol extract of Asparagus racemosus was found to possess the highest total antioxidant capacity (639.925±64.78) mg/gm followed by methanol (616.92±53.88) mg/gm and petroleum ether (469.17±52.95) mg/gm ascorbic acid equivalent respectively. In brine shrimp lethality bioassay, LC50 values for ethanol, methanol, petroleum ether, n-hexane and chloroform were found to be 0.674 µg/ml, 0.719 µg/ml, 0.984 µg/ml, 2.157µg/ml and 1.514 µg/ml respectively. N-hexane and chloroform extract showed least activity in all the measures. The results suggest that Asparagus racemosus is a valuable source  of antioxidant and has significant cytotoxic activity hence could eliminate many diseases related to free radical.

DOI: http://dx.doi.org/10.3329/icpj.v1i9.11615

International Current Pharmaceutical Journal 2012, 1(9): 250-257

 

Downloads

Download data is not yet available.
Abstract
113
PDF
107

Downloads

Published

2012-08-04

How to Cite

Hossain, M. I., Sharmin, F. A., Akhter, S., Bhuiyan, M. A., & Shahriar, M. (2012). Investigation of cytotoxicity and in-vitro antioxidant activity of Asparagus racemosus root extract. International Current Pharmaceutical Journal, 1(9), 250–257. https://doi.org/10.3329/icpj.v1i9.11615

Issue

Vol. 1 No. 9 (2012)

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

  1. The journal holds copyright and publishes the work under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).