Development and validation of RP-HPLC method for the estimation of omeprazole in bulk and capsule dosage forms

Authors

  • Kalakonda Sri Nataraj Shri Vishnu College of Pharmacy, Bhimavaram-534202, West Godavari District, Andhra Pradesh
  • Mohammad Badrud Duza Shri Vishnu College of Pharmacy, Bhimavaram-534202, West Godavari District, Andhra Pradesh
  • Kalyani Pragallapati Shri Vishnu College of Pharmacy, Bhimavaram-534202, West Godavari District, Andhra Pradesh
  • Dussa Kiran Kumar Center for Pharmaceutical Sciences, JNTUH, Hyderabad -500072, Andhra Pradesh

DOI:

https://doi.org/10.3329/icpj.v1i11.12062

Keywords:

Omeprazole, RP-HPLC, determination, capsule dosage form, C18, linearity

Abstract

A method for the determination of omeprazole in bulk and capsule dosage form by reverse phase high performance liquid chromatography has been developed. This is a simple, rapid, precise and an accurate method. The method was developed on a Novapak C18, (250 x 4.6 mm, 5µ) column using phosphate buffer (pH 7.4) and acetonitrile in the ratio of 60:40, v/v as a mobile phase which was pumped at a flow rate of 1.0 ml/min and detection was done at 302 nm. The retention time of the drug was found to be 7.71 min. The method was validated for accuracy, precision, linearity, specificity, robustness. The linearity was observed in the range of 20-60 ppm. The results of recovery studies indicated that the method was accurate. Hence the developed method was found to be suitable for the estimation of omeprazole in bulk and capsule dosage forms.

DOI: http://dx.doi.org/10.3329/icpj.v1i11.12062

International Current Pharmaceutical Journal 2012, 1(11): 366-369

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Published

2012-10-03

How to Cite

Nataraj, K. S., Duza, M. B., Pragallapati, K., & Kumar, D. K. (2012). Development and validation of RP-HPLC method for the estimation of omeprazole in bulk and capsule dosage forms. International Current Pharmaceutical Journal, 1(11), 366–369. https://doi.org/10.3329/icpj.v1i11.12062

Issue

Vol. 1 No. 11 (2012)

Section

Original Articles

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