RP-HPLC analysis for the simultaneous estimation of rabeprazole sodium and aceclofenac in a combined dosage form

Authors

  • Sukhbir Lal Khokra Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra, Haryana
  • Balram Choudhary Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra, Haryana
  • Heena Mehta Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra, Haryana

DOI:

https://doi.org/10.3329/icpj.v1i12.12450

Keywords:

Quantitative analysis, validation, capsule, standard addition method, anti-inflammatory, analgesic

Abstract

A rapid, simple and highly sensitive reversed phase high performance liquid chromatographic (RP-HPLC) method has been developed for the quantitative determination of Rabeprazole sodium and Aceclofenac in a combined dosage form. Rabeprazole sodium and Aceclofenac were chromatographed using C-18 column as stationary phase and methanol: acetonitrile: water (60 : 10 : 30 v/v/v) as the mobile phase at a flow rate of 1.0 ml/min at ambient temperature and detected at 280 nm. The retention time (RT) of Rabeprazole sodium and Aceclofenac were found to be 5.611 min and 2.102 minute, respectively. The linearities of Rabeprazole sodium and Aceclofenac were in the range of 1-10 µg/ml and 3-15 µg/ml, respectively. The limit of detection was found to be 0.091 µg/ml for Rabeprazole sodium and 0.043 µg/ml for Aceclofenac. The proposed method was applied for the determination of Rabeprazole sodium and Aceclofenac in a combined dosage form and result was found satisfactory.

DOI: http://dx.doi.org/10.3329/icpj.v1i12.12450

International Current Pharmaceutical Journal 2012, 1(12): 410-413

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Published

2012-11-01

How to Cite

Khokra, S. L., Choudhary, B., & Mehta, H. (2012). RP-HPLC analysis for the simultaneous estimation of rabeprazole sodium and aceclofenac in a combined dosage form. International Current Pharmaceutical Journal, 1(12), 410–413. https://doi.org/10.3329/icpj.v1i12.12450

Issue

Vol. 1 No. 12 (2012)

Section

Original Articles

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