Evaluation of cytotoxicity, anti-herpes simplex virus type 1 (HSV-1) and antibacterial activities of Ficus vasta and phytoconstituents

Authors

  • Khaled Rashed National Research Centre Pharmacognosy Department Dokki, Giza
  • Lucy Ono Yasuyoshi Hayashi Microbiology Laboratory Department of Basic Pathology UFPR, Curitiba, PR

DOI:

https://doi.org/10.3329/icpj.v3i1.17295

Keywords:

Plant extract, biological activities, cytotoxicity, anti-HSV-1, antibacterial, phytoconstituents

Abstract

This study deals with the evaluation of in vitro cytotoxicity of the methanol 80% extract of Ficus vasta aerial parts in Vero cells, anti-HSV-1 and antibacterial activities and the determination of the phytochemical content of the F. vasta methanol extract. The extract was tested for its cytotoxicity in Vero cells and for anti-HSV-1 and against bacterial strains as S. aureus, S. epidermidis, E. coli and P. aeruginosa and also phytochemical constituents were detected. The results showed that this extract presented a CC50 value of 389 µg/mL, an intermediate value taking into account other researchers reported for some Moraceae species. Regarding the other biological activities, antiviral effect was not demonstrated by a HSV-1 challenge dilution of 100x tissue culture infective dose 50% (TCID50) and also by the disk-diffusion method at the higher non-toxic concentration of the extract (119 µg/mL). Phytochemical analysis of the methanol extract proved the presence of carbohydrates, tannins, flavonoids, coumarins, alkaloids and triterpenes. Chromatographic separation of the methanol extract resulted in the isolation and identification of ?-sitosterol, stigmasterol, lupeol, ursolic acid, naringenin, luteolin, quercetin, vitexin, quercetin 3-O-?-glucoside and rutin. The results indicated that F. vasta aerial parts methanol extract is potential candidate for experiments of biological activity screening that are not anti-HSV-1 or antibacterial against the strains evaluated in this study, to be carried on in the future.

DOI: http://dx.doi.org/10.3329/icpj.v3i1.17295

International Current Pharmaceutical Journal, December 2013, 3(1): 211-218

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Published

2013-12-09

How to Cite

Rashed, K., & Ono, L. (2013). Evaluation of cytotoxicity, anti-herpes simplex virus type 1 (HSV-1) and antibacterial activities of Ficus vasta and phytoconstituents. International Current Pharmaceutical Journal, 3(1), 211–218. https://doi.org/10.3329/icpj.v3i1.17295

Issue

Vol. 3 No. 1 (2013)

Section

Original Articles

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