Streptomyces: isolation, optimization of culture conditions and extraction of secondary metabolites

Authors

  • Ahmed I. Khattab Microbiology Department Al-Yarmouk College, Khartoum
  • Eltahir H. Babiker Department of Biochemistry Faculty of Medicine and Health Science University of El Imam El Mahadi
  • Humodi A. Saeed College of Medical Laboratory Science Sudan University of Science and Technology Khartoum

DOI:

https://doi.org/10.3329/icpj.v5i3.26695

Keywords:

Streptomyces, Red Sea, antibacterial activity, GC-MS, Sudan

Abstract

The objectives of this study were to isolate and identify Streptomyces from soil sediments as well as to optimize cultural growth conditions for maximum antibacterial productivity. A total of fifty soil sediments were collected from Red Sea, Sudan. The soil sediments were pretreated and cultivated on agar medium. Promising Streptomyces spp. were isolated by agar overlay method using indicator organisms. Optimization of chemical and physical culture conditions was carried out. The later was judged by assessment of antibacterial activity. Ethyl acetate was used to extract the secondary metabolite compounds. The separation of the active ingredients was performed using both thin layer chromatography (TLC) and gas chromatography-mass spectrometer (GC-MS). The results revealed nine strains of Streptomyces. Of them two (PS1 and PS28) isolates exhibited high activity against pathogenic bacteria. The optimum growth conditions were pH 7.5, temperature at 30°C, soyabean concentration 2.5 g/l, incubation period in 7 days, MgSO4.7H2O conc. 1g/l and K2HPO4 conc. 2.5g/l. TLC test showed three and two fragments from metabolites of PS1 and PS28 respectively, while the GC-MS analysis revealed eight and eleven compounds with antibacterial activity of PS1 and PS28 respectively. It is concluded that marine is promising source of secondary metabolites.

Khattab et al., International Current Pharmaceutical Journal, February 2016, 5(3): 27-32

Downloads

Download data is not yet available.
Abstract
255
PDF
546

Downloads

Published

2016-02-06

How to Cite

Khattab, A. I., Babiker, E. H., & Saeed, H. A. (2016). Streptomyces: isolation, optimization of culture conditions and extraction of secondary metabolites. International Current Pharmaceutical Journal, 5(3), 27–32. https://doi.org/10.3329/icpj.v5i3.26695

Issue

Vol. 5 No. 3 (2016)

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

  1. The journal holds copyright and publishes the work under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).