Development of Efficient Callus Initiation of Malta (Citrus sinensis) Through Tissue Culture
DOI:
https://doi.org/10.3329/ijarit.v1i1-2.13935Keywords:
Malta, Citrus sinensis, In vitro, Seed shoot formation, Callus inductionAbstract
The effects of different hormonal concentration on shoot formation and callus induction were studied on BARI Malta-1 (Citrus sinensis). Seeds containing seed coat and without seed coat was treated by Murashige and Skoog (MS) media supplemented with 6-benzyl adenine (BA) and Kinetin (KIN). Removal of seed coat showed an early response for shoot formation. The highest (70%) shoot formation was obtained from seeds without seed coat treated with MS basal media + BA 1.0 mg/l while KIN showed no response for shoot formation in any supplemented concentration. However, in case of callus induction internodes and apical shoot tips were used as explants and 2, 4-dichlorophenoxy acetic acid (2, 4-D) was used as callus inducing hormone. MS basal media supplemented with 2, 4-D, 2.0 mg/l showed highest (68%) callus induction.
DOI: http://dx.doi.org/10.3329/ijarit.v1i1-2.13935
Int. J. Agril. Res. Innov. & Tech. 1 (1&2): 64-68, December, 2011
Downloads
280
437
Downloads
Published
How to Cite
Issue
Section
License
- Copyright on any research article is transferred in full to the International Journal of Agricultural Research, Innovation and Technology upon publication in the journal. The copyright transfer includes the right to reproduce and distribute the article in any form of reproduction (printing, electronic media or any other form).
- Articles in the International Journal of Agricultural Research, Innovation and Technology are Open Access articles published under the Creative Commons CC BY License (https://creativecommons.org/licenses/by/4.0/)
- This license permits use, distribution and reproduction in any medium, provided the original work is properly cited.