Diversity and dynamics of rhizobial populations in acidic soils with aluminum and manganese toxicities in forest zones

Authors

  • Linda Manet Department of Microbiology, Faculty of Science, University of Yaounde I. P.O. Box: 812 Yaounde and Department of Plant Biology, Faculty of Science, University of Yaounde I. P.O. Box: 812 Yaounde
  • Onana Boyomo Department of Microbiology, Faculty of Science, University of Yaounde I. P.O. Box: 812 Yaounde
  • Eddy Léonard Mangaptche Ngonkeu Department of Microbiology, Faculty of Science, University of Yaounde I. P.O. Box: 812 Yaounde and Department of Plant Biology, Faculty of Science, University of Yaounde I. P.O. Box: 812 Yaounde
  • Aimé Didier Boyogueno Begoudé Biological Control and Applied Microbiology Laboratory, IRAD, P.O.Box:2067
  • Papa Saliou Sarr Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji 611-0011

DOI:

https://doi.org/10.3329/ijarit.v6i2.31700

Keywords:

Acidity, Al and Mn Toxicity, Rhizobia, Diversity, PCR-RFLP

Abstract

Soil acidity in the humid forest zones of Cameroon is one of the major constraints to agricultural productivity. This study was carried out to assess the rhizobial communities of two acidic soils; with aluminum toxicity (Nkoemvone) and manganese toxicity (Nkolbisson) for their potential to improve soil fertility in Cameroon. These two soils were used to inoculate to the host plants cowpea and siratro. At harvest, 120 rhizobacterial isolates were extracted from the nodules of these two hosts and subjected to morphological characterization. Twenty isolates per site were selected and analyzed for their 16S rDNA genetic profile following restrictions with endonucleases of PCR products and electrophoresis. The restriction patterns of the 16S rDNA of the 40 isolates showed 12 different profiles. Eight occurred in both types of soils, where as 4 were specific to the manganese-toxic-acidic soil. While the Al toxicity reduced the nodulation and growth of both plants, the Mn toxicity mostly affect the cowpea. This study ascertained the distribution of rhizobia based on soil characteristics. Further molecular analyses would allow the identification of the isolates recovered as well as their phylogenetical relationships.

Int. J. Agril. Res. Innov. & Tech. 6 (2): 12-23, December, 2016

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Author Biography

Linda Manet, Department of Microbiology, Faculty of Science, University of Yaounde I. P.O. Box: 812 Yaounde and Department of Plant Biology, Faculty of Science, University of Yaounde I. P.O. Box: 812 Yaounde



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Published

2017-02-27

How to Cite

Manet, L., Boyomo, O., Ngonkeu, E. L. M., Begoudé, A. D. B., & Sarr, P. S. (2017). Diversity and dynamics of rhizobial populations in acidic soils with aluminum and manganese toxicities in forest zones. International Journal of Agricultural Research, Innovation and Technology, 6(2), 12–23. https://doi.org/10.3329/ijarit.v6i2.31700

Issue

Vol. 6 No. 2 (2016)

Section

Articles

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