Simultaneous Determination of Clopidogrel and Pioglitazone by High Performance Liquid Chromatography in Bulk Drug and Dosage Forms

Authors

  • V Phani Kumar Department of chemistry, Acharya Nagarjuna University, Guntur, Andhra Pradesh
  • Y Sunandamma Department of chemistry, Acharya Nagarjuna University, Guntur, Andhra Pradesh

DOI:

https://doi.org/10.3329/ijpls.v2i1.14580

Keywords:

Clopidogrel, Pioglitazone, HPLC-UV method, bioavailability, 230 nm

Abstract

A high performance liquid chromatographic method with UV detection was developed and validated for simultaneous determination of Pioglitazone and Clopidogrel. Separation was performed on a C18 column by isocratic elution with a mobile phase of Methanol: Acetonitrile: Water (80:10:10) at pH 4.6. The UV detection was set at 230 nm. The method proved to be specific, accurate, precise and linear over the concentration ranges of 20-120ppm for both Pioglitazone and Clopidogrel with correlation coefficients always >0.999 for both drugs. The intra-day and inter-day precision and accuracy were less than 2 for both analytes.

DOI: http://dx.doi.org/10.3329/ijpls.v2i1.14580

International Journal of Pharmaceutical and Life Sciences Vol.2(1) 2013: 1-9

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Published

2013-04-15

How to Cite

Kumar, V. P., & Sunandamma, Y. (2013). Simultaneous Determination of Clopidogrel and Pioglitazone by High Performance Liquid Chromatography in Bulk Drug and Dosage Forms. International Journal of Pharmaceutical and Life Sciences, 2(1), 1–9. https://doi.org/10.3329/ijpls.v2i1.14580

Issue

Section

Research Articles