Isolation and molecular detection of Pasteurella multocida Type A from naturally infected chickens, and their histopathological evaluation in artificially infected chickens in Bangladesh

Authors

  • Sayedun Nahar Panna Department of Microbiology and Hygiene, FVS, BAU, Mymensingh
  • K.H.M. Nazmul Hussain Nazir Department of Microbiology and Hygiene, FVS, BAU, Mymensingh
  • M. Bahanur Rahman Department of Microbiology and Hygiene, FVS, BAU, Mymensingh
  • Sultan Ahamed Department of Microbiology and Hygiene, FVS, BAU, Mymensingh
  • Md. Golam Saroare Business Development Officer, Chars Livelihoods Program
  • Shovon Chakma One Health Epidemiology Fellow, Massey University
  • Tazrin Kamal Department of Microbiology and Hygiene, FVS, BAU, Mymensingh
  • Ummay Habiba Majumder Department of Microbiology and Hygiene, FVS, BAU, Mymensingh

Keywords:

Chicken, Fowl cholera, Histopathology, Pasteurella multocida type A, PCR

Abstract

Pasteurella multocida type A is the etiologic agent of fowl cholera, a highly contagious and fatal disease of chickens. The present research work was performed for the isolation, identification and molecular detection of P. multocida Type A from chickens. Liver, heart and spleen of suspected dead chicken (n=35) were collected from Gazipur and Pabna districts in Bangladesh. The targeted bacteria from the samples were isolated, identified and characterized based on their morphology, staining, cultural, biochemical characters, pathogenicity test, histopathological study and Polymerase Chain Reaction (PCR). The P. multocida organism was isolated from 11.42% (n=4/35) samples. The organisms were gram negative, non-spore forming rod, non-motile, occurring singly or pairs in Gram staining, whereas in Leishmans stain, bipolar shaped organisms were observed. All the isolates were found positive for oxidase and catalase tests, produced indole, and fermented glucose, mannitol and sucrose. Necrotic foci in liver and congestion with hemorrhages in heart were found on necropsy. After pathogenicity test, the pathological changes were reconfirmed by histopathology depicting congestion, hemorrhage and lymphocyte infiltration in heart, liver and spleen tissues. In type specific PCR reaction, the organisms were confirmed as P. multocida Type A. In conclusion, P. multocida type A is prevalent among poultry in the studied regions; thus, care must be taken to control of the disease.

 http://dx.doi.org/10.5455/javar.2015.b104


Downloads

Download data is not yet available.
Abstract
1220
PDF
1149

Downloads

Published

2015-09-07

How to Cite

Panna, S. N., Nazir, K. N. H., Rahman, M. B., Ahamed, S., Saroare, M. G., Chakma, S., Kamal, T., & Majumder, U. H. (2015). Isolation and molecular detection of Pasteurella multocida Type A from naturally infected chickens, and their histopathological evaluation in artificially infected chickens in Bangladesh. Journal of Advanced Veterinary and Animal Research, 2(3), 338–345. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/24871

Issue

Vol. 2 No. 3 (2015)

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).