Сharacterization of lytic activity of Phage SAvB14 on Staphylococcus aureus variant bovis

Authors

  • Yulia Horiuk Faculty of Veterinary Medicine and Technologies in Livestock, Department of Infectious and Parasitic Diseases, State Agrarian and Engineering University in Podilya, Kamianets-Podilskyi, Ukraine.
  • Victor Horiuk Faculty of Veterinary Medicine and Technologies in Livestock, Department of Veterinary Obstetrics, Pathology and Surgery, State Agrarian and Engineering University in Podilya, Kamianets-Podilskyi, Ukraine.
  • Mykola Kukhtyn Faculty of Engineering of Machines, Structures and Technologies, Department of Food Biotechnology and Chemistry, Ternopil Ivan Pului National Technical University, Ternopil, Ukraine.
  • Anatoliy Tsvihun Faculty of Veterinary Medicine and Technologies in Livestock, Department of Animal Feeding, Breeding and Feed Technology, State Agrarian and Engineering University in Podilya, Kamianets-Podilskyi, Ukraine.
  • Sergiy Kernychnyi Faculty of Veterinary Medicine and Technologies in Livestock, Department of Veterinary Obstetrics, Pathology and Surgery, State Agrarian and Engineering University in Podilya, Kamianets-Podilskyi, Ukraine.

Keywords:

Phage; staphylococci; mastitis, phage infection, lytic activity

Abstract

Objective: The objective of this study was to investigate the intensity of phage infection caused by Phage SAvB14, which was isolated from dairy farms, depending on the initial number of Staphylococcus aureus cells in the medium.  Material and methods: To evaluate the impact of the viable bacteria S. aureus var. bovis on the intensity of phage infection caused by Phage SAvB14, 1 mg of phagolysate (phage titer 105 CFU/ ml) was introduced in 9 ml of nutrient broth with an appropriate amount of daily culture of S. aureus var. bovis under study. The number of viable staphylococci was determined by total viable count/ml.  Results: In this experiment, we found that the intensity of phages lytic activity was dependent on the number of sensitive bacterial cells in the volume of the culture medium. Effective phage ther­apy requires a high concentration of phages in the medium (inflammation foci) for rapid contact of the virus with bacteria.  Conclusion: When developing a phage drug to treat subclinical mastitis, it is necessary to increase the phage titer in the drug or its dosage compared to the clinical form, as there is a lower proba­bility of phage contact with a susceptible microbial cell. Besides, at a high concentration of bacte­ria, there is a gradual decrease in nutrients in the medium, resulting in phages going back to the condition of lysogeny.

J. Adv. Vet. Anim. Res., 7(3): 509-513, Sep 2020

http://doi.org/10.5455/javar.2020.g447

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Published

2020-08-20

How to Cite

Horiuk, Y., Horiuk, V., Kukhtyn, M., Tsvihun, A., & Kernychnyi, S. (2020). Сharacterization of lytic activity of Phage SAvB14 on Staphylococcus aureus variant bovis. Journal of Advanced Veterinary and Animal Research, 7(3), 509–513. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/75685

Issue

Vol. 7 No. 3 (2020)

Section

Short Communications

License

Copyright (c) 2020 Yulia Horiuk, Victor Horiuk, Mykola Kukhtyn, Anatoliy Tsvihun, Sergiy Kernychnyi

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