Isolation of multidrug-resistant Escherichia coli from turkeys in Dinajpur, Bangladesh, and their antibiogram profile

Authors

  • Tonmoy Kundu Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Rangpur, Bangladesh.
  • Nazmi Ara Rumi Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Rangpur, Bangladesh.
  • Md Khaled Hossain Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Rangpur, Bangladesh.
  • Md Shajedur Rahman Department of Medicine, Surgery and Obstetrics, Hajee Mohammad Danesh Science and Technology University, Rangpur, Bangladesh.
  • Mirdha Md Kamal Hossain Animal Biotechnology Division, National Institute of Biotechnology, Ashulia, Bangladesh.
  • Joyanta Halder Animal Biotechnology Division, National Institute of Biotechnology, Ashulia, Bangladesh.

Keywords:

Antimicrobial resistance; cloacal swabs; Escherichia coli; fecal swabs; Turkey

Abstract

Objective: The study was carried out for molecular characterization and antibiotic resistance anal­ysis of Escherichia coli isolated from different turkey farms in Dinajpur, Bangladesh.  Materials and methods: A total of 45 samples comprising feces (n = 23) and cloacal swabs (n = 22) were collected randomly from turkeys. The samples were subjected to isolation and identification of E. coli by cultural and biochemical characteristics, followed by polymerase chain reaction and sequencing. An antibiogram of the isolated E. coli isolates was carried out by following the Kirby– Bauer disk diffusion method.  Results: Out of the 45 samples, 28 (62.21%) were positive for E. coli, of which 16 (35.55%) fecal samples were positive and 12 (26.66%) cloacal swabs were positive . The antibiotic sensitivity analysis revealed that all the E. coli isolates were 100% sensitive to levofloxacin, norfloxacin, neo­mycin, gentamicin, and nitrofurantoin. On the other hand, all the isolates were 100% resistant to amoxicillin, azithromycin, erythromycin, tetracycline, bacitracin, cephalexin, nalidixic acid, van­comycin, methicilin, piperacillin, pefloxacin, novobiocin, cefepime, trimethoprim, netilmicin, and aztreonam.  Conclusion: This study’s results uncover the occurrence and antibiotic resistance pattern of E. coli in the study area’s turkeys.

J. Adv. Vet. Anim. Res., 8(1): 64-69, Mar 2021

http://doi.org/10.5455/javar.2021.h486

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Published

2021-03-05

How to Cite

Kundu, T., Rumi, N. A., Hossain, M. K., Rahman, M. S., Hossain, M. M. K., & Halder, J. (2021). Isolation of multidrug-resistant Escherichia coli from turkeys in Dinajpur, Bangladesh, and their antibiogram profile. Journal of Advanced Veterinary and Animal Research, 8(1), 64–69. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/76012

Issue

Vol. 8 No. 1 (2021)

Section

Original Articles

License

Copyright (c) 2021 Tonmoy Kundu, Nazmi Ara Rumi, Md Khaled Hossain, Md Shajedur Rahman, Mirdha Md Kamal Hossain, Joyanta Halder

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