Bacterial loads and antibiotic resistance profile of bacteria isolated from the most popular street food (Phuchka) in Bangladesh

Authors

  • Mahadi Hasan Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
  • Farzana Siddika Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
  • Md Arefin Kallol Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
  • Najibullah Sheikh Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
  • Muhammad Tofazzal Hossain Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
  • Md Mahmudul Alam Department of Surgery and Obstetrics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
  • Marzia Rahman Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Keywords:

Street food; Phuchka; Bacterial load; Antibiotic resistance

Abstract

Objective: Phuchka is one of the most common street foods in Bangladesh. It is served with salad, sweet and sour tamarind dispersed water, and minced eggs as topping at places where people usually gather. This makes these foods susceptible to bacterial contamination. Therefore, assess­ing the bacterial load and antimicrobial profile of organisms isolated from phuchka and other foodstuffs served with it was the focus of this study.  Materials and Methods: Bacterial isolates were isolated and identified from the samples after the bacterial loads were assessed as total viable count (TVC), total coliform count (TCC), and total staphylococcal count (TSC). The antibiotic resistance profile of the isolates was obtained using the disk diffusion method. Molecular detection of Escherichia coli isolates and the presence of gene responsible for tetracycline resistance was confirmed by polymerase chain reaction.  Results: According to the recommendations, the TVC value of 70% phuchka and egg samples was safe, whereas TSC value illustrated that 80% of both phuchka and egg samples were at safety level. For the TCC value, 80% egg and 70% phuchka samples were found to be safe for consump­tion. Among all the samples, the microbial loads of the vendors’ hand wash were least in the safety level. Antibiotic sensitivity tests revealed that both Staphylococcus spp. and E. coli isolates were sensitive to gentamicin and ciprofloxacin but showed resistance to ampicillin.  Conclusion: The data of this study indicate that phuchka can pose a public health problem as foodborne bacterial isolates which are antibiotic-resistant are found in it.

J. Adv. Vet. Anim. Res., 8(3): 361-366, September 2021

http://doi.org/10.5455/javar.2021.h523

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Published

2021-07-03

How to Cite

Hasan, M., Siddika, F., Kallol, M. A., Sheikh, N., Hossain, M. T., Alam, M. M., & Rahman, M. (2021). Bacterial loads and antibiotic resistance profile of bacteria isolated from the most popular street food (Phuchka) in Bangladesh. Journal of Advanced Veterinary and Animal Research, 8(3), 361–366. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/76370

Issue

Vol. 8 No. 3 (2021)

Section

Original Articles

License

Copyright (c) 2021 Mahadi Hasan, Farzana Siddika, Md Arefin Kallol, Najibullah Sheikh, Muhammad Tofazzal Hossain, Md Mahmudul Alam, Marzia Rahman

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