First case report on molecular detection of Trypanosoma lewisi in an urban rat in Kelantan, Malaysia: An accidental finding

Authors

  • Intan Noor Aina Kamaruzaman Department of Paraclinical Studies, Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bharu, Kelantan, Malaysia.
  • Hong Wei Ting Department of Clinical Studies, Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bharu, Kelantan, Malaysia.
  • Muhammad Aiman Mohd Mokhtar Department of Clinical Studies, Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bharu, Kelantan, Malaysia.
  • Yong Kai Yuan Department of Clinical Studies, Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bharu, Kelantan, Malaysia.
  • Azim Wafiy Gulam Shah Department of Clinical Studies, Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bharu, Kelantan, Malaysia2Department of Clinical Studies, Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bharu, Kelantan, Malaysia.
  • Fathin Faahimaah Abdul Hamid Department of Paraclinical Studies, Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bharu, Kelantan, Malaysia.
  • Che Wan Salma Che Wan Zalati Department of Paraclinical Studies, Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bharu, Kelantan, Malaysia.
  • Nurshahirah Shaharulnizim Department of Clinical Studies, Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bharu, Kelantan, Malaysia.
  • Mohd Farhan Hanif Reduan Department of Clinical Studies, Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bharu, Kelantan, Malaysia.
  • Luqman Abu Bakar Department of Paraclinical Studies, Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bharu, Kelantan, Malaysia.

Keywords:

Kelantan; polymerase chain reaction; rats; Trypanosoma lewisi

Abstract

Objective: This case report highlights the first detection of Trypanosoma lewisi, a blood protozoan parasite found in an urban rat in Kota Bharu, Kelantan.  Materials and Methods: Rat trapping was carried out within the Kota Bharu vicinity near a local wet market. A total of 38 rats were captured and subjected to peripheral blood smearing using Giemsa stain. Positive rats were sent for histopathological analysis for the evaluation of the organ samples.  Results: The presence of trypanosomes was found in one sample from a blood smear. This was connected to a histological lesion on kidney tissues, which revealed a high concentration of trypanosomes. Additionally, the positive sample was confirmed as T. lewisi based on molecular diagnosis via polymerase chain reaction and subsequent sequencing and phylogenetic analysis.  Conclusions: This finding serves as a baseline for further surveillance on T. lewisi population among urban rats in Kelantan and possible zoonotic transmission to humans.

J. Adv. Vet. Anim. Res., 8(2): 435-439, June 2021

http://doi.org/10.5455/javar.2021.h531

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Published

2021-09-19

How to Cite

Kamaruzaman, I. N. A., Ting, H. W., Mokhtar, M. A. M., Yuan, Y. K., Shah, A. W. G., Hamid, F. F. A., Zalati, C. W. S. C. W., Shaharulnizim, N., Reduan, M. F. H., & Bakar, L. A. (2021). First case report on molecular detection of Trypanosoma lewisi in an urban rat in Kelantan, Malaysia: An accidental finding. Journal of Advanced Veterinary and Animal Research, 8(3), 435–439. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/76393

Issue

Vol. 8 No. 3 (2021)

Section

Case Reports

License

Copyright (c) 2021 Intan Noor Aina Kamaruzaman, Hong Wei Ting, Muhammad Aiman Mohd Mokhtar, Yong Kai Yuan, Azim Wafiy Gulam Shah, Fathin Faahimaah Abdul Hamid, Che Wan Salma Che Wan Zalati, Nurshahirah Shaharulnizim, Mohd Farhan Hanif Reduan, Luqman Abu Bakar

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