Validation of portable electronic equipment (Accutrend® Plus) to determine glucose, total cholesterol, and triglycerides in rats (Rattus) and dogs (Canis lupus familiaris)

Authors

  • Paola de la Paz Ramírez Facultad de Medicina, Veterinaria y Zootecnia, Universidad Michoacana de San Nicolas de Hidalgo, Michoacán, México. https://orcid.org/0000-0002-0859-2333
  • Gerardo Ordaz Centro Nacional de Investigación Disciplinaria en Fisiología y Mejoramiento Animal-INIFAP, Querétaro, México. https://orcid.org/0000-0003-4502-3727
  • Reynaldo de la Paz Gonzáles Facultad de Ciencias Médicas y Bilógicas Dr. “Ignacio Chávez,” Universidad Michoacana de San Nicolas de Hidalgo, Michoacán, México.
  • Rosa Elena Pérez Facultad de Químico Farmacobiología, Universidad Michoacana de San Nicolas de Hidalgo, Michoacán, México. https://orcid.org/0000-0003-2895-7384
  • Manuel López Facultad de Medicina, Veterinaria y Zootecnia, Universidad Michoacana de San Nicolas de Hidalgo, Michoacán, México. https://orcid.org/0000-0002-5226-5356
  • Ruy Ortiz Facultad de Medicina, Veterinaria y Zootecnia, Universidad Michoacana de San Nicolas de Hidalgo, Michoacán, México. https://orcid.org/0000-0002-5226-5356

Keywords:

Blood chemistry; concordance; linearity; correlation

Abstract

Objective: This study aimed to validate the analytical precision of the Accutrend® Plus portable electronic equipment (PE) to determine glucose (GLU), total cholesterol (TC), and triglycerides (TG) in rats and dogs using the conventional laboratory method (CM) as a reference. Materials and Methods: To determine the analytical accuracy of the Accutrend® Plus in the measurement of GLU, CT, and TG. The EP-9-A2 guide (Clinical and Laboratory Standards Institute), Bland-Altman graphical analysis, and Lin’s correlation coefficient of concordance (CCC) were implemented. Results: The average differences (p > 0.05) between PE and CM for GLU, TC, and TG were 2.21, 1.20, and 0.72 mg·dl−1, respectively, in rats and 1.06, 4.30, and 2.41 mg·dl−1, respectively, in dogs (p > 0.05). Both methods showed a linear relationship with Pearson’s correlation coefficients > 0.96 and R2 > 0.97 for the three biochemical indicators evaluated in both species. The GLU, TC, and TG values obtained by the PE were substantial, as evident from Lin’s CCC > 0.96. Conclusion: The PE Accutrend® Plus is potent for monitoring GLU, TC, and TG in rats and dogs because of its precision and ability to facilitate measurement by reducing stress in animals during sampling.

J. Adv. Vet. Anim. Res., 10(1): 57–63, March 2023

http://doi.org/10.5455/javar.2023.j652

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Published

2023-03-31

How to Cite

Ramírez, P. de la P., Ordaz, G., Gonzáles, R. de la P., Pérez, R. E., López, M., & Ortiz, R. (2023). Validation of portable electronic equipment (Accutrend® Plus) to determine glucose, total cholesterol, and triglycerides in rats (Rattus) and dogs (Canis lupus familiaris). Journal of Advanced Veterinary and Animal Research, 10(1), 57–63. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/80414

Issue

Vol. 10 No. 1 (2023)

Section

Original Articles

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Copyright (c) 2023 Paola de la Paz Ramírez, Gerardo Ordaz, Reynaldo de la Paz Gonzáles, Rosa Elena Pérez, Manuel López, Ruy Ortiz

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