Collagen properties of Indonesian local sheepskin isolated using acid and enzymatic methods.

Authors

  • Dita Prameswari Trenggono Putri Department of Animal Products Technology, Faculty of Animal Science, Gadjah Mada University, Yogyakarta, Indonesia. https://orcid.org/0009-0001-5704-0017
  • Vernanda Widya Pangestika Department of Animal Products Technology, Faculty of Animal Science, Gadjah Mada University, Yogyakarta, Indonesia.
  • Hanifan Ilyas Department of Animal Products Technology, Faculty of Animal Science, Gadjah Mada University, Yogyakarta, Indonesia.
  • Mohammad Zainal Abidin Department of Animal Products Technology, Faculty of Animal Science, Gadjah Mada University, Yogyakarta, Indonesia.
  • Nanung Agus Fitriyanto Department of Animal Products Technology, Faculty of Animal Science, Gadjah Mada University, Yogyakarta, Indonesia.
  • Yuny Erwanto Department of Animal Products Technology, Faculty of Animal Science, Gadjah Mada University, Yogyakarta, Indonesia; Institute for Halal Industry and System, Gadjah Mada University, Yogyakarta, Indonesia. https://orcid.org/0000-0002-6139-9698

Keywords:

Enzymatic method; Acid method; Collagen; Characteristics; Garut Sheep.

Abstract

Objectives: This study aimed to investigate the physical properties of Indonesian local sheep skin collagen extracted by acid and enzymatic methods. Materials and Methods: Collagen was isolated from Pure Breed Garut Sheep (Ovis aries sp.) skin, 1.5 years old. The skins were obtained from a local slaughterhouse in Cirebon, Indonesia. The solvents used were CH3COOH and three different enzymes: neutrase, alcalase, and bromelain. Results: The highest yields of extracted collagen were bromelain-soluble collagen (BSC), which reached 37.07%. The range of Ph values for all samples started from 4.01 to 4.76. The viscosity values (cP) of acid-soluble collagen (ASC), neutrase-soluble collagen (NSC), alcalase-soluble collagen (LSC), and BSC were 3.42, 3.90, 3.45, and 3.12, respectively. Regarding SDS-PAGE analyses, Garut sheepskin collagen is categorized as collagen type I, which has a molecular weight of about 140.99 to 148.74 kDa for α1 and around 110 to 111.86 kDa for α2. The results of FTIR and DSC analyses for all samples show the same motif with commercial collagen motifs based on the literature. Conclusion: Garut sheep skin has the potential to be an alternative raw material source for producing collagen. Collagen extracted using a combination of CH3COOH and bromelain enzyme showed the most desirable results in almost every characteristic.

J. Adv. Vet. Anim. Res., 11(3): 722–728, September 2024

http://doi.org/10.5455/javar.2024.k823

Downloads

Download data is not yet available.
Abstract
10
PDF
13

Downloads

Published

2024-09-29

How to Cite

Putri, D. P. T., Pangestika, V. W., Ilyas, H., Abidin, M. Z., Fitriyanto, N. A., & Erwanto, Y. (2024). Collagen properties of Indonesian local sheepskin isolated using acid and enzymatic methods. Journal of Advanced Veterinary and Animal Research, 11(3), 722–728. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/81056

Issue

Vol. 11 No. 3 (2024)

Section

Original Articles

License

Copyright (c) 2024 Dita Prameswari Trenggono Putri, Vernanda Widya Pangestika, Hanifan Ilyas, Mohammad Zainal Abidin, Nanung Agus Fitriyanto, Yuny Erwanto

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).