Genetic diversity and association analysis of the growth hormone gene in local Indonesian chicken

Authors

  • Nurul Pratiwi Research Center for Animal Husbandry, National Research and Innovation Agency of the Republic of Indonesia (BRIN), Cibinong, Bogor, Indonesia. https://orcid.org/0000-0001-5334-5772
  • Nurul Azizah Research Center for Animal Husbandry, National Research and Innovation Agency of the Republic of Indonesia (BRIN), Cibinong, Bogor, Indonesia. https://orcid.org/0000-0003-0076-3596
  • Anne Sukmara Directorate of Laboratory Management, Research Facilities, and Science and Technology Park, National Research and Innovation Agency of the Republic of Indonesia (BRIN), Bogor, Indonesia. https://orcid.org/0009-0000-8406-3874
  • Bayu Dewantoro Putro Soewandi Research Center for Animal Husbandry, National Research and Innovation Agency of the Republic of Indonesia (BRIN), Cibinong, Bogor, Indonesia. https://orcid.org/0000-0001-8413-2729
  • Latifah Latifah Research Center for Animal Husbandry, National Research and Innovation Agency of the Republic of Indonesia (BRIN), Cibinong, Bogor, Indonesia. https://orcid.org/0009-0007-4692-5008
  • Tuti Haryati Research Center for Animal Husbandry, National Research and Innovation Agency of the Republic of Indonesia (BRIN), Cibinong, Bogor, Indonesia. https://orcid.org/0000-0002-0839-3051
  • Bram Brahmantiyo Research Center for Animal Husbandry, National Research and Innovation Agency of the Republic of Indonesia (BRIN), Cibinong, Bogor, Indonesia. https://orcid.org/0000-0001-5095-3852
  • Jonathan Anugrah Lase Research Center for Animal Husbandry, National Research and Innovation Agency of the Republic of Indonesia (BRIN), Cibinong, Bogor, Indonesia. https://orcid.org/0000-0003-0053-8794
  • Dwi Rohmadi Directorate of Laboratory Management, Research Facilities, and Science and Technology Park, National Research and Innovation Agency of the Republic of Indonesia (BRIN), Bogor, Indonesia. https://orcid.org/0009-0006-9839-8134
  • Usman Usman Research Center for Animal Husbandry, National Research and Innovation Agency of the Republic of Indonesia (BRIN), Cibinong, Bogor, Indonesia. https://orcid.org/0009-0001-8249-5224
  • Paskah Partogi Agung Research Center for Applied Zoology, National Research and Innovation Agency of the Republic of Indonesia (BRIN), Bogor, Indonesia. https://orcid.org/0000-0003-2554-1893
  • Maijon Purba Research Center for Animal Husbandry, National Research and Innovation Agency of the Republic of Indonesia (BRIN), Cibinong, Bogor, Indonesia. https://orcid.org/0009-0008-2350-1955
  • Mohamad Farid Ridhillah Directorate of Laboratory Management, Research Facilities, and Science and Technology Park, National Research and Innovation Agency of the Republic of Indonesia (BRIN), Bogor, Indonesia. https://orcid.org/0009-0003-9940-3706
  • Abdullah Baharun Department of Animal Science, Faculty of Agriculture, Universitas Djuanda, Bogor, Indonesia. https://orcid.org/0000-0002-3688-8844
  • Ferdy Saputra Research Center for Animal Husbandry, National Research and Innovation Agency of the Republic of Indonesia (BRIN), Cibinong, Bogor, Indonesia. https://orcid.org/0000-0002-7949-9777
  • Tike Sartika Research Center for Animal Husbandry, National Research and Innovation Agency of the Republic of Indonesia (BRIN), Cibinong, Bogor, Indonesia. https://orcid.org/0000-0002-1354-3229

Keywords:

Carcass traits; genetic diversity; growth hormone; Indonesian chickens; laying traits

Abstract

Objective: The aim of this study was to investigate the effects of the Growth hormone gene, including its additive and dominant components, on laying performance (age at first laying, body weight at first laying, weight of the first egg, total eggs produced by 24 weeks, and total eggs produced by 48 weeks) and carcass attributes (live weight, carcass weight, breast weight, back weight, thigh weight, and wing weight). Materials and Methods: A total of 35 Kampung hens and 19 Sentul roosters were used in this study. Genotyping at the GH|MspI and GH|SacI loci was conducted using the restriction fragment length polymorphism (RFLP) method. Association analyses were conducted using analysis of variance, while additive and dominance effects were assessed through generalized linear models implemented in R version 4.3.1. Results: RFLP analysis revealed polymorphism at the GH|MspI locus but not at GH|SacI. No significant differences in any trait were observed among the GH|MspI genotypes. Similarly, no significant additive or dominance effects were detected. Moreover, nanopore sequencing identified two novel single-nucleotide polymorphisms, g.3242delG and g.3396_3401del, in both Kampung and Sentul chickens. Conclusion: No significant association was found between these polymorphisms and laying or carcass traits. Additionally, no significant additive or dominance effects were found, implying that these loci did not significantly influence the definition of these traits in Kampung and Sentul chickens.

J. Adv. Vet. Anim. Res., 12(4): 1151–1158, December 2025

http://doi.org/10.5455/javar.2025.l976

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Published

2026-04-22

How to Cite

Pratiwi, N., Azizah, N., Sukmara, A., Soewandi, B. D. P., Latifah, L., Haryati, T., … Sartika, T. (2026). Genetic diversity and association analysis of the growth hormone gene in local Indonesian chicken. Journal of Advanced Veterinary and Animal Research, 12(4), 1151–1158. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/88541

Issue

Vol. 12 No. 4 (2025)

Section

Original Articles

License

Copyright (c) 2025 Nurul Pratiwi, Nurul Azizah, Anne Sukmara, Bayu Dewantoro Putro Soewandi, Latifah Latifah, Tuti Haryati, Bram Brahmantiyo, Jonathan Anugrah Lase, Dwi Rohmadi, Usman Usman, Paskah Partogi Agung, Maijon Purba, Mohamad Farid Ridhillah, Abdullah Baharun, Ferdy Saputra, Tike Sartika

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