Effect of Doping Agent on Elution Profile of Tc-99m Generationand Labeling of Tc-99m With

Abstract

Effect of doping agent on elution profile of Tc-99m generation and labeling of Tc-99m with MDP (Methylene diphosphonate) were carried out and studied. Tc -99m is one of the most talked element of modern radio pharmacy for its multiple application in diagnosis.Tc-99m is produced from Mo-99 through radioactive decay process. In our work two column chromatographic generator were made with Alumina column and loaded with Mo-99 which would be converted into Tc-99m eventually. Elution was taken by passing saline( 1 mL 0.9% NaCl solution and 1 mL 0.9% NaCl solution with 0.0045% NaNO₃ as doping agent) each time and the activities of Tc -99m was measured by a Dose Calibrator. Elutions of Tc-99m were carried out several times and each time activity was measured. It is observed that almost 60 -75 % activity can be found in 2^nd elution. Studying elution profile it is seen that activity curve reaches at pick in 2^nd elution and afterwards gradually slopes down. Each time better activity is achieved by using pure saline. In spite of lower activity, this research resulting highly recommendation of using NaNO₃ as a doping agent in NaCl saline in case of using elution because of its higher purity and RCP value . Tc -99m is applied over a biological body as a labeled compound . In this case MDP was used as a labeling compound . With a view of carrying out the RCP value of labeled compound, paper chromatography technique is done with various mobile phases in Whatman No 1 paper. Purity (%) is ranging from 30 90 % and NaNO₃ doped NaCl radioactive solution shows better purity performance than pure NaCl solution in every different mobile phase .

DOI: http://dx.doi.org/10.3329/jce.v27i2.17800

Journal of Chemical Engineering, IEB Vol. ChE. 27, No. 2, December 2012: 41-45