Gene Xpert a Promising Tool in Diagnosis of Extrapulmonary TB in Developing Countries

Authors

  • Jabin Akhter Associate Professor, Dept of Microbiology. NIPSOM, Dhaka, Bangladesh
  • MA Hassan Chowdhury Director, Bangladesh Institute of Tropical and Infectious Diseases, Fouzderhat, Chittagong, Bangladesh

DOI:

https://doi.org/10.3329/jopsom.v39i1.51859

Keywords:

EPTB, Gene Xpert, diagnosis

Abstract

Introduction: Although Tuberculosis mostly affects lungs in about 85% cases, but can cause lesion almost in every part of the body. Extrapulmonary TB (EPTB) accounts for 15 to 20% which involves other parts of the body beside the lungs. There are several methods that can diagnose Pulmonary TB (PTB) conclusively , but extrapulmonary TB is very difficult to diagnose till now especially in resource limited settings. Though it is not communicable but diagnostic delay has made it significant cause of morbidity and mortality. The snidy was aimed to find out the Gene Xpert as one of the diagnostic tool for EPTB.

Methods: A laboratory based descriptive cross sectional study was conducted over a period of  17  months  from January 2017 to May 2018 to ascertain the performance of Gene Xpert technique as a diagnostic tool for EPTB. Data were collected through checklist and a total of 77 clinical samples were collected purposively with prior infom,ed consent from suspected EPTB patients following ethical issues. Laboratory investigations were  perfomed  at Rhodolphe Merieux Laboratory, Chittagong, Bangladesh with Gene Xpert MTB/Ri f assay, conventional culture (LJ media) and Microscopy (ZN stain) for the presence of Mycobacteium tuberculosis (MTB).

Results: Among the 77 samples from suspected cases, seven(9.09%) from CSF, one(!.29%) from pus and one(l .29%) from lymphnode specimens were positive by Gene Xpert MTB/Rif assay.Only one(1.29%) CSF specimen  was found to be culture and microscopy positive which was Gene Xpert positive also. Except one specimen from pus that is both Gene Xpert and microscopy positive but culture negative, no other specimens from EPTB cases were culture and microscopy positive.

Conclusion: Diagnosis of EPTB is challenging in worldwide. As it is paucibacillary, routine diagnostic test in detecting MTB is difficult. Gene X pert showed promising outcome in early detection of life threatening EPTB cases like TB meningitis which is common in developing countries.

JOPSOM 2020; 39(1): 26-30

Downloads

Download data is not yet available.
Abstract
35
PDF
51

Downloads

Published

2021-02-04

How to Cite

Akhter, J., & Chowdhury, M. H. (2021). Gene Xpert a Promising Tool in Diagnosis of Extrapulmonary TB in Developing Countries. Journal of Preventive and Social Medicine, 39(1), 26–30. https://doi.org/10.3329/jopsom.v39i1.51859

Issue

Vol. 39 No. 1 (2020)

Section

Original Articles

License

Copyright Notice

Authors who publish in the Journal of Preventive and Social Medicine (JOPSOM) agree to the following terms that:

  1. Authors retain copyright and grant the Journal of Preventive and Social Medicine (JOPSOM) the right of first publication of the work.

  2. Articles in the Journal of Preventive and Social Medicine (JOPSOM) are Open Access articles published under the Creative Commons CC BY-NC License  Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). This license permits Share — copy and redistribute the material in any medium or format and Adapt — remix, transform, and build upon the material.