A Comparative Study of LAMP and PCR in Relation to Time and Cost
DOI:
https://doi.org/10.3329/jssmc.v12i2.56885Keywords:
Loop-mediated isothermal amplification, qPCR, Low cost, Short-time operation.Abstract
Background: The Loop-mediated isothermal amplification (LAMP) represents a very sensitive, easy to use, and less time consuming diagnostic method.
Aims: The aim was to establish a simple, cost-effective, molecular technique.
Materials and methods: An analytical study was conducted using two hundred acute serum samples using two different molecular techniques; qPCR and LAMP to standardize a costeffective and less time-consuming technique.
Results: The cost of in-house LAMP reagents was one-ninth of the cost of commercial qPCR. Consume cost was 23 times less than qPCR besides, lab setup cost was 92 times less than qPCR. More importantly, LAMP requires 5-6 times less time duration than qPCR.
Conclusion: Due to its simple short-time operation with low cost, it would be a prevalent molecular technique globally, particularly in Bangladesh.
J Shaheed Suhrawardy Med Coll 2020; 12(2): 72-75
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