In vitro Regeneration of Grass Pea (Lathyrus sativus L.)

Authors

  • P Saha Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Tangail-1902
  • M Afrin Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Tangail-1902
  • AKM Mohiuddin Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Tangail-1902
  • AM Shohael Department of Biotechnology and Genetic Engineering, Jahangirnagar University, Savar, Dhaka-1342

DOI:

https://doi.org/10.3329/jujbs.v4i2.27790

Keywords:

Acclimatization, direct shoot regeneration, grass pea, organogenesis, root initiation

Abstract

In vitro regeneration protocol for grass pea (Lathyrus sativus L.) was optimized using different concentrations and combinations of growth regulators. Direct shoot regeneration obtained through shoot organogenesis from different explants of grass pea cultured on MS medium supplemented with Gamborg B5 vitamin containing 6-benzylaminopurine (BAP), Thidiazuron (TDZ) and ?-naphthalene acetic acid (NAA). Highest percentage of shoots were obtained at 4.0 mg/l of BAP on nodal explants. Stunted multiple shoots were developed from nodal explants while 1.5 mg/l TDZ was used. About 56% of direct shoots were also obtained, while the combination of BAP (4.0 mg/l) and NAA (0.5 mg/l) were used. Regenerated plantlets were rooted most effectively (40%) in rooting medium containing half strength of MS basal medium containing 1.0 mg/l NAA. Well rooted plantlets were further successfully acclimatized to ambient humidity level and grown in controlled environment until hardening.

Jahangirnagar University J. Biol. Sci. 4(2): 1-8, 2015 (December)

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Author Biography

P Saha, Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Tangail-1902



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Published

2016-05-18

How to Cite

Saha, P., Afrin, M., Mohiuddin, A., & Shohael, A. (2016). In vitro Regeneration of Grass Pea (Lathyrus sativus L.). Jahangirnagar University Journal of Biological Sciences, 4(2), 1–8. https://doi.org/10.3329/jujbs.v4i2.27790

Issue

Vol. 4 No. 2 (2015)

Section

Original Research Articles

License

(c) Jahangirnagar University Journal of Biological Sciences.  

Articles in the Jahangirnagar University Journal of Biological Sciences are Open Access articles published under the Creative Commons CC BY License (https://creativecommons.org/licenses/by/4.0/). This license permits use, distribution and reproduction in any medium, provided the original work is properly cited.