Optimization of culture condition, phytoconstituents and cytotoxicity of Xylaria hypoxylon (L.) Grev.

Abstract

Experiments were carried out to determine the optimum culture condition, phytochemical constituents as well as cytotoxic activity of the endophytic fungus Xylaria hypoxylon (L.) Grev. grown on rotten bamboo poles at Jahangirnagar University campus. The fungus was first identified up to the species level, then suitable growth medium under laboratory condition was ascertained. Among the different types of culture media, Potato dextrose agar (PDA) medium was found to be the most suitable for optimum growth of this fungus at pH 6 and 30ºC. Phytochemical study of fruit body extract showed substantial presence of terpenoids, steroids, phenolic compounds, and weak presence of glycosides, falvonoids, and saponins. Brine shrimp lethality assay of the extract showed moderate cytotoxicity with LC₅₀ value 327.00 μg/ml after 6 hours.

Jahangirnagar University J. Biol. Sci. 6(2): 1-9, 2017 (December)