Variability among selective guava (Psidium guajava L.) varieties revealed by morphology and RAPD marker

Authors

  • Farzana Alam Department of Genetic Engineering and Biotechnology, Jagannath University, Dhaka-1100, Bangladesh
  • Kazi Didarul Islam Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh
  • SM Mahbubur Rahman Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh

DOI:

https://doi.org/10.3329/jujbs.v7i2.40750

Keywords:

Polymorphic, genetic diversity, RAPD, Psidium guajava L.

Abstract

The research was conducted for the assessment of genetic diversity using both morphological and random amplified polymorphic DNA (RAPD) analysis of twelve guava (Psidium guajava L.) varieties growing in Bangladesh. Morphological characterization of guava varieties showed a wide range of variation. The highest variability was observed between Poly and Jelly varieties.Polymerase chain reaction with 5 arbitrary 10-mer and 3 arbitrary 12- mer RAPD primers produced a total of 50 bands of which 75.23 percent were polymorphic. The highest percentage of polymorphic loci (100%) was observed for primer A and the lowest (50%) for A03 primer. The UPGMA dendrogram revealed the segregation pattern and the difference of evolutionary changes. Guava varieties were separated into two main groups, one of them was made up of Chineese, Jelly, Kazi, Apple, L-49, Local-2 and Local-3. The other one was made up of Local-1, Poly, Kashi, Thai and Bombay. The highest genetic distance between Apple and Kazi peyara indicate that these varieties might be interesting in breeding programme for improving trait of interest. This scientific information could be used for further improvement of guava.

Jahangirnagar University J. Biol. Sci. 7(2): 89-98, 2018 (December)

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Published

2018-12-30

How to Cite

Alam, F., Islam, K. D., & Rahman, S. M. (2018). Variability among selective guava (Psidium guajava L.) varieties revealed by morphology and RAPD marker. Jahangirnagar University Journal of Biological Sciences, 7(2), 89–98. https://doi.org/10.3329/jujbs.v7i2.40750

Issue

Vol. 7 No. 2 (2018)

Section

Original Research Articles

License

(c) Jahangirnagar University Journal of Biological Sciences.  

Articles in the Jahangirnagar University Journal of Biological Sciences are Open Access articles published under the Creative Commons CC BY License (https://creativecommons.org/licenses/by/4.0/). This license permits use, distribution and reproduction in any medium, provided the original work is properly cited.