Infectivity Assays and Sequence Analyses for Unassigned Pseudomonas Species as Putative Cause of Dieback Disease of Dalbergia sissoo Roxb. in Bangladesh

Authors

  • Hanny Tantau Molecular Phytopathology and Genetics, Biocentre Klein Flottbek, University of Hamburg, Hamburg
  • Steffi Renk Molecular Phytopathology and Genetics, Biocentre Klein Flottbek, University of Hamburg, Hamburg
  • Dorothee Schultz Molecular Phytopathology and Genetics, Biocentre Klein Flottbek, University of Hamburg, Hamburg
  • Heidrun Meyer Molecular Phytopathology and Genetics, Biocentre Klein Flottbek, University of Hamburg, Hamburg
  • Jana Schulze Molecular Phytopathology and Genetics, Biocentre Klein Flottbek, University of Hamburg, Hamburg
  • Denise Palm Molecular Phytopathology and Genetics, Biocentre Klein Flottbek, University of Hamburg, Hamburg
  • Annika Stubbe Molecular Phytopathology and Genetics, Biocentre Klein Flottbek, University of Hamburg, Hamburg
  • Annika Stubbe Molecular Phytopathology and Genetics, Biocentre Klein Flottbek, University of Hamburg, Hamburg
  • Nayuf Valdez Aguirre Molecular Phytopathology and Genetics, Biocentre Klein Flottbek, University of Hamburg, Hamburg
  • Rakha Hari x Sarker Department of Botany, University of Dhaka, Dhaka-1000
  • Sk. Shamimul Alam Department of Botany, University of Dhaka, Dhaka-1000
  • Mihir Lal Saha Department of Botany, University of Dhaka, Dhaka-1000
  • M. Salim Khan 2BCSIR Laboratories, Tissue Culture Section, Dhaka-1205
  • M. Imdadul Hoque Department of Botany, University of Dhaka, Dhaka-1000
  • Hans-Peter Mühlbach Molecular Phytopathology and Genetics, Biocentre Klein Flottbek, University of Hamburg, Hamburg

DOI:

https://doi.org/10.3329/ptcb.v21i2.10214

Keywords:

Sissoo, Dieback, Pseudomonas, Fungal pathogens

Abstract

identified unequivocally. Putative microbial pathogens (fungi and bacteria) were studied in dieback affected sissoo trees collected from Bangladesh, using plant pathological techniques combined with molecular tools. DNA based characteri-zation showed the presence of heterogeneous patterns of various fungi (mostly saprophytic). It did not support the hypothesis of Fusarium solani being the cause of sissoo dieback. In contrast, isolation and molecular characterisation of bacteria from dieback affected sissoo revealed the presence of Pseudomonas in 83% of the samples. Sequencing the gene of 16S ribosomal RNA, the rpoD-gene, the gacA-gene and the rnpB-gene strongly suggested that these isolates belong most probably to a still unassigned Pseudomonas species. Hypersensitivity response assays and infection studies using sissoo seedlings demonstrated their pathogenic potential.

 

Key words: Sissoo, dieback, Pseudomonas, fungal pathogens

 

D.O.I. 10.3329/ptcb.v21i2.10214

 

Plant Tissue Cult. & Biotech. 21(2): 101-113, 2011 (December)

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Published

2011-12-31

How to Cite

Tantau, H., Renk, S., Schultz, D., Meyer, H., Schulze, J., Palm, D., Stubbe, A., Stubbe, A., Aguirre, N. V., Sarker, R. H. x, Alam, S. S., Saha, M. L., Khan, M. S., Hoque, M. I., & Mühlbach, H.-P. (2011). Infectivity Assays and Sequence Analyses for Unassigned Pseudomonas Species as Putative Cause of Dieback Disease of Dalbergia sissoo Roxb. in Bangladesh. Plant Tissue Culture and Biotechnology, 21(2), 101–113. https://doi.org/10.3329/ptcb.v21i2.10214

Issue

Vol. 21 No. 2 (2011)

Section

Articles