Somatic Embryogenesis Induction in Narcissus papyraceus cv. Shirazi

Authors

  • Saghi Anbari No. 63, 10th Floor, 21 Building Omid Shahrak, Ghanat Kosar, Tehran, Iran
  • Mosoud Tohidfar Seed and Plant Improvement Campus, Mahdasht Road, Karaj, Iran.
  • Ramin Hosseini International University of Imam Khomeini, Ghazvin, Iran.
  • Rahim Haddad International University of Imam Khomeini, Ghazvin, Iran.

Keywords:

Narcissus, Somatic embryogenesis, Regeneration

Abstract

Explants from bulb scales of Narcissus papyraceus cv. Shirazi cultured in MS and Nitsch media containing different concentrations of BAP, 2,4-D, GA3, Kn, IBA and NAA produced highest number of regenerated bulblets in Nitsch medium containing BAP (2.2 mg/l) and 1.1 mg/l 2,4-D. The highest number of direct somatic embryos were observed in MS containing 0.5 mg/l GA3, 1.6 mg/l BAP and 1.6 mg/l 2,4-D. Induced somatic embryos were multiplied by transferring them to hormone free MS.

Key words: Narcissus, Somatic embryogenesis, Regeneration

DOI = 10.3329/ptcb.v17i1.1119

Plant Tissue Cult. & Biotech. 17(1): 37-46, 2007 (June)

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Somatic Embryogenesis Induction in Narcissus papyraceus cv. Shirazi. (2008). Plant Tissue Culture and Biotechnology, 17(1), 37-46. https://doi.org/10.3329/ptcb.v17i1.1119

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How to Cite

Somatic Embryogenesis Induction in Narcissus papyraceus cv. Shirazi. (2008). Plant Tissue Culture and Biotechnology, 17(1), 37-46. https://doi.org/10.3329/ptcb.v17i1.1119