Detection of Maize Intrinsic and Recombinant <i>Cry1Ab</i> Gene Fragment in Genetically Modified Maize

Authors

  • T Rahman Department of Biotechnology, Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh, Bangladesh.
  • EH Chowdhury Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh
  • AC Mondol Department of Biotechnology, Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh, Bangladesh.
  • MM Hoque Department of Biotechnology, Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh, Bangladesh.
  • KM Nasiruddin Department of Biotechnology, Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh, Bangladesh.

DOI:

https://doi.org/10.3329/ptcb.v17i1.1127

Keywords:

Detection, Cry1Ab gene, Maize intrinsic gene, GM maize

Abstract

Polymerase chain reaction (PCR) assay was performed to detect and identify the genetically modified maize (Bt11). Primer pair Bt11 1-5´ and Cry1Ab 1-3´ detected the region of the insect resistant Cry1Ab gene sequence inserted in GM Bt11 maize and a primer pair ZE01-ZE02 were used to detect the maize intrinsic zein (Ze1) gene in maize DNA. The presence of the corresponding DNA segments was specifically detected in GM maize by the designed primers. It was concluded that this method is useful for fast and easy screening of Bt gene in the food products and GM Bt crops.

Key words: Detection, Cry1Ab gene, Maize intrinsic gene, GM maize

DOI = 10.3329/ptcb.v17i1.1127

Plant Tissue Cult. & Biotech. 17(1): 103-108, 2007 (June)

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How to Cite

Rahman, T., Chowdhury, E., Mondol, A., Hoque, M., & Nasiruddin, K. (2008). Detection of Maize Intrinsic and Recombinant <i>Cry1Ab</i> Gene Fragment in Genetically Modified Maize. Plant Tissue Culture and Biotechnology, 17(1), 103–108. https://doi.org/10.3329/ptcb.v17i1.1127

Issue

Vol. 17 No. 1 (2007)

Section

Short Communications