In vitro Seed Germination and Seedling Development of Esmeralda clarkei Rchb.f. (Orchidaceae)

Authors

  • M Paudel Central Department of Botany Bhairahawa Multiple Campus, Tribhuvan University, Bhairahawa, Rupandehi
  • S Pradhan Central Department of Botany Bhairahawa Multiple Campus, Tribhuvan University, Bhairahawa, Rupandehi
  • B Pant Central Department of Botany Bhairahawa Multiple Campus, Tribhuvan University, Bhairahawa, Rupandehi

DOI:

https://doi.org/10.3329/ptcb.v22i2.14197

Keywords:

Esmeralda clarkei, In vitro, Protocorm, Seedling

Abstract

Esmeralda clarkei Rchb.f. (Orchidaceae), an epiphytic orchid native to Nepal with high ornamental value grows from cool to intermediate climatic conditions with medium amount of light. In an attempt to preserve this important orchid by establishing an efficient in vitro regeneration protocol using seed culture was conducted. MS with BAP and NAA alone or in combination were used. In MS protocorms developed within 16 weeks followed by seedling development in 25 weeks of seed culture. Presence of BAP (0.5 mg/l) in MS improved seed germination by protocorm formation and seedling development in 17 and 26 weeks of seed culture, respectively. In 0.5 mg/l NAA supplemented media, development of protocorms was observed in 18 weeks and seedling growth was observed in 26 weeks of seed culture. Protocorm and seedling development were was delayed in other media.

DOI: http://dx.doi.org/10.3329/ptcb.v22i2.14197

Plant Tissue Cult. & Biotech. 22(2): 107-111, 2012 (December)

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Published

2013-03-19

How to Cite

Paudel, M., Pradhan, S., & Pant, B. (2013). In vitro Seed Germination and Seedling Development of Esmeralda clarkei Rchb.f. (Orchidaceae). Plant Tissue Culture and Biotechnology, 22(2), 107–111. https://doi.org/10.3329/ptcb.v22i2.14197

Issue

Vol. 22 No. 2 (2012)

Section

Articles