Authentication of Silybum marianum Varieties Using RAPD Analysis

Authors

  • Sameh Abou Zid Pharmacognosy Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62111

DOI:

https://doi.org/10.3329/ptcb.v24i1.19200

Keywords:

Silybum marianum, Authentication, RAPD analysis

Abstract

Silybum marianum is an important medicinal plant used for hepatic disorders. Two varieties exist for this plant S. marianum var. album (white-flowered) and S. marianum var. purple (purple-flowered). A fast and simple method (RAPD) was developed for authentication of S. marianum varieties (purple- and white- flowered) at a DNA level. The two varieties were distinguished by polymorphic bands generated by four decamer primers, namely, OPP-10, OPG-03, OPG-01, and OPC-17. The developed RAPD method will be useful for practical authentication of S. marianum varieties and their adulterants.

Plant Tissue Cult. & Biotech. 24(1): 57-63, 2014 (June)

D. O. I. http://dx.doi.org/10.3329/ptcb.v24i1.19200

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Published

2014-06-19

How to Cite

Zid, S. A. (2014). Authentication of Silybum marianum Varieties Using RAPD Analysis. Plant Tissue Culture and Biotechnology, 24(1), 57–63. https://doi.org/10.3329/ptcb.v24i1.19200

Issue

Vol. 24 No. 1 (2014)

Section

Articles