In vitro regeneration and somatic embryogenesis in citrus

Authors

  • El Sawy A Mohamed Department of Pomology, Faculty of Agriculture, Caitro University, Giza
  • Amina Gomaa Department of Pomology, Faculty of Agriculture, Caitro University, Giza
  • Nancy Danial Plant Biotechnology Department, National Research Center, Dokki, Giza

DOI:

https://doi.org/10.3329/ptcb.v24i2.23558

Keywords:

Citrus, In vitro regeneration, Somatic embryogenesis

Abstract

Better results were obtained when stigma explants of variegated lemon and citron were used. After ten months, somatic embryos developed into plantlets at a frequency ranged from 13.3 for lime to 66.7% for lemon. Virus presence was tested by ELISA and RT?PCR. The results indicated that the plantlets regenerated through somatic embryogenesis are CTV?free. RAPD analysis was used to asses the genetic stability of plantlets as compared to the mother plants. The results indicated that most plantlets belong to the respective mother plants and the polymorphism percentage was genotype and explant?dependant.

Plant Tissue Cult. & Biotech. 24(2): 247-262, 2014 (December

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Author Biography

El Sawy A Mohamed, Department of Pomology, Faculty of Agriculture, Caitro University, Giza



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Published

2015-06-02

How to Cite

Mohamed, E. S. A., Gomaa, A., & Danial, N. (2015). In vitro regeneration and somatic embryogenesis in citrus. Plant Tissue Culture and Biotechnology, 24(2), 247–262. https://doi.org/10.3329/ptcb.v24i2.23558

Issue

Vol. 24 No. 2 (2014)

Section

Articles