Morphological and Genetic Characterization of Micropropagated Field Grown Plants of Aloe vera L.

Authors

  • Anusree Das Department of Genetics, University of Calcutta, Kolkata?700019
  • SK Moquammel Haque Department of Botany, RKMVC College, Rahara, Kolkata? 700118
  • Biswajit Ghosh Department of Botany, RKMVC College, Rahara, Kolkata? 700118
  • Krishnadas Nandagopal Department of Genetics, University of Calcutta, Kolkata?700019
  • Timir Baran Jha PG Department of Botany, Barasat Govt. College, Barasat, Kolkata?700124

DOI:

https://doi.org/10.3329/ptcb.v25i2.26257

Keywords:

Aloe vera, Fruit and fertile seed production, Genetic characterization, Somaclone

Abstract

A large scale shoot multiplication from apical meristem in Aloe vera L. was obtained on MS with 35.5 ?M BAP, 9.8 ?M IBA and 81.4 ?M adenine sulphate. Fifty micropropagated plants were successfully transferred to the field and maintained to attain reproductive phase. Field evaluation of micropropagated plants is important to assess predicted clonal fidelity. Exo-morphological evaluation of Aloe plants, identified three seed setting plants, designated as somaclones. Seeds were viable and germinated (70.58%) in vitro. Although chromosome number and morphology of somaclones were identical with the donor plants their RAPD profiles and ITS-1 sequences were different from donor plant. This study reports Seed setting somaclones in Aloe vera, for the first time which may serve as new genetic resource for biotechnological improvement.

Plant Tissue Cult. & Biotech. 25(2): 231-246, 2015 (December)

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Author Biography

Anusree Das, Department of Genetics, University of Calcutta, Kolkata?700019



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Published

2016-01-04

How to Cite

Das, A., Haque, S. M., Ghosh, B., Nandagopal, K., & Jha, T. B. (2016). Morphological and Genetic Characterization of Micropropagated Field Grown Plants of Aloe vera L. Plant Tissue Culture and Biotechnology, 25(2), 231–246. https://doi.org/10.3329/ptcb.v25i2.26257

Issue

Vol. 25 No. 2 (2015)

Section

Articles