In vitro Shoot Multiplication of Withania coagulans (Stocks) Dunal

Authors

  • Harsh Joshi P.G. Department of Biosciences, Sardar Patel University, Sardar Patel Maidan, Vadtal Road, Satellite Campus, Post Box No. 39, Vallabh Vidyanagar?388120
  • Sureshkumar Nekkala P.G. Department of Biosciences, Sardar Patel University, Sardar Patel Maidan, Vadtal Road, Satellite Campus, Post Box No. 39, Vallabh Vidyanagar?388120
  • Deepak Soner P.G. Department of Biosciences, Sardar Patel University, Sardar Patel Maidan, Vadtal Road, Satellite Campus, Post Box No. 39, Vallabh Vidyanagar?388120
  • Mafatlal M Kher P.G. Department of Biosciences, Sardar Patel University, Sardar Patel Maidan, Vadtal Road, Satellite Campus, Post Box No. 39, Vallabh Vidyanagar?388120
  • M Nataraj P.G. Department of Biosciences, Sardar Patel University, Sardar Patel Maidan, Vadtal Road, Satellite Campus, Post Box No. 39, Vallabh Vidyanagar?388120

DOI:

https://doi.org/10.3329/ptcb.v26i2.30569

Keywords:

Shoot multiplication, Withania coagulans, Hyperhydricity

Abstract

Withania coagulans (Stocks) Dunal is an important medicinal plant of Solanaceae. Nodal segments obtained from field grown plants were used as explants. 1 ? 2 cm long nodal segment with a single one bud was cultured on MS containing 2.5 mg/l thidiazuron (TDZ), 0.1 mg/l NAA and 50 mg/l adenine sulphate (AdS) resulted in formation of 5.16 shoots per node. However, vitrification was observed in all explants within one month. On the other hand nodal explants cultured on MS supplemented with 2.50 mg/l meta?topoline (mT) with 0.1 mg/l NAA and 50 mg/l AdS resulted in the formation of 4.50 healthy and uniformly grown shoots per node.

Plant Tissue Cult. & Biotech. 26(2): 187-195, 2016 (December)

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Author Biography

Harsh Joshi, P.G. Department of Biosciences, Sardar Patel University, Sardar Patel Maidan, Vadtal Road, Satellite Campus, Post Box No. 39, Vallabh Vidyanagar?388120



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Published

2016-12-10

How to Cite

Joshi, H., Nekkala, S., Soner, D., Kher, M. M., & Nataraj, M. (2016). In vitro Shoot Multiplication of Withania coagulans (Stocks) Dunal. Plant Tissue Culture and Biotechnology, 26(2), 187–195. https://doi.org/10.3329/ptcb.v26i2.30569

Issue

Vol. 26 No. 2 (2016)

Section

Articles