Genetic Diversity Assessment of Luffa aegyptiaca Landraces Endemic in Egypt Based on Some Molecular Markers

Authors

  • Mohammad Abdel Sttar Al Tahlawy Plant Biotechnology Department, Genetic Engineering and Biotechnology Division, National Research Centre, 33 El Bohouth St. (former El?Tahrir St.), Dokki, Giza P.O. 12622
  • Mahmoud Abdel Aziz Ibrahim Molecular Biology Department, Genetic Engineering and Biotechnology Division, National Research Centre, 33 El Bohouth St. (former El? Tahrir St.), Dokki, Giza P.O. 12622
  • Mohamed Ahmed Matter Plant Biotechnology Department, Genetic Engineering and Biotechnology Division, National Research Centre, 33 El Bohouth St. (former El?Tahrir St.), Dokki, Giza P.O. 12622,
  • Mervat El Sayed Mohamed Chemistry Department, Faculty of Science, Cairo University, Giza
  • Mahmoud Mohamed Sakr Plant Biotechnology Department, Genetic Engineering and Biotechnology Division, National Research Centre, 33 El Bohouth St. (former El?Tahrir St.), Dokki, Giza P.O. 12622,

DOI:

https://doi.org/10.3329/ptcb.v26i2.30571

Keywords:

Genetic diversity, Luffa aegyptiaca, Endemic landraces, Molecular markers

Abstract

Luffa aegyptiaca is a popular climbing herb endemic in Egypt. We studied the genetic diversity among ten Luffa landraces (Cairo, Beni Suef, Menoufiya, Damietta, Banha, Aswan, Kafr el?Sheikh, Bir el?Abd, MarsaMatruh and Asyut) collected from different districts in Egypt. The results obtained from DNA fingerprinting revealed that there were polymorphic loci with average percentage of 44.6 among collected landraces whereas polymorphic loci obtained from SDS?PAGE were 23%. Discrimination between landraces was more efficient by using RAPD?PCR marker than total proteins SDS?PAGE which showed a limited level of intraspecific diversity.

Plant Tissue Cult. & Biotech. 26(2): 209-217, 2016 (December)

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Author Biography

Mohammad Abdel Sttar Al Tahlawy, Plant Biotechnology Department, Genetic Engineering and Biotechnology Division, National Research Centre, 33 El Bohouth St. (former El?Tahrir St.), Dokki, Giza P.O. 12622



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Published

2016-12-10

How to Cite

Al Tahlawy, M. A. S., Ibrahim, M. A. A., Matter, M. A., Mohamed, M. E. S., & Sakr, M. M. (2016). Genetic Diversity Assessment of Luffa aegyptiaca Landraces Endemic in Egypt Based on Some Molecular Markers. Plant Tissue Culture and Biotechnology, 26(2), 209–217. https://doi.org/10.3329/ptcb.v26i2.30571

Issue

Vol. 26 No. 2 (2016)

Section

Articles