Procedure to Identify and Submit cDNA Sequences to

Abstract

With rapid advances in DNA sequencing and large collection of such information available, it may be helpful to have a simplified procedure for analysis and submission of such sequence information. The process has been described in three steps: (a) data gathering and preparation, (b) data analysis, and (c) data submission to Genbank. The use of NCBI-tools such as VecScreen and ORF finder has been shown to be effective in detecting the vector contaminated sequences and in identifying the segments that code for protein with ‘start-’ and ‘stop’ codon, respectively. Another software called ‘A Plasmid Editor’ is useful in reconstructing the complete DNA sequence of a gene from the sequences of complementary strand. The process of sequence data submission to the GenBank about the gene of interest is described in an easy-to-follow manner, including the final step of receiving an accession number from NCBI.

Key words: Jute cDNA library, Corchorus olitorius, VecScreen, ORF Finder, Vector Contamination, GenBank, BankIt, Heat shock protein, TAIR WUBLAST

D.O.I. 10.3329/ptcb.v17i2.3236

Plant Tissue Cult. & Biotech. 17(2): 161-172, 2007 (December)