Procedure to Identify and Submit cDNA Sequences to
DOI:
https://doi.org/10.3329/ptcb.v17i2.3236Keywords:
Jute cDNA library, Corchorus olitorius, VecScreen, ORF Finder, Vector Contamination, GenBank, BankIt, Heat shock protein, TAIR WUBLASTAbstract
With rapid advances in DNA sequencing and large collection of such information available, it may be helpful to have a simplified procedure for analysis and submission of such sequence information. The process has been described in three steps: (a) data gathering and preparation, (b) data analysis, and (c) data submission to Genbank. The use of NCBI-tools such as VecScreen and ORF finder has been shown to be effective in detecting the vector contaminated sequences and in identifying the segments that code for protein with ‘start-’ and ‘stop’ codon, respectively. Another software called ‘A Plasmid Editor’ is useful in reconstructing the complete DNA sequence of a gene from the sequences of complementary strand. The process of sequence data submission to the GenBank about the gene of interest is described in an easy-to-follow manner, including the final step of receiving an accession number from NCBI.
Key words: Jute cDNA library, Corchorus olitorius, VecScreen, ORF Finder, Vector Contamination, GenBank, BankIt, Heat shock protein, TAIR WUBLAST
D.O.I. 10.3329/ptcb.v17i2.3236
Plant Tissue Cult. & Biotech. 17(2): 161-172, 2007 (December)
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