Re-evaluation of Mature Seed-derived Callusing and Regeneration Potential of Nine Orchardgrass (Dactylis golomerata L.) Cultivars

Authors

  • Sang-Hoon Lee Present address: National Institute of Animal Sciences, 77 Chuksan Gil, Suwon 441-706
  • Nagib Ahsan Division of Applied Life Sciences (BK21), Gyeongsang National University,
  • Ki-Won Lee Division of Applied Life Sciences (BK21), Gyeongsang National University,
  • Dong-Gi Lee Division of Applied Life Sciences (BK21), Gyeongsang National University,
  • Iftekhar Alam Division of Applied Life Sciences (BK21), Gyeongsang National University,
  • Kyung-Hee Kim Division of Applied Life Sciences (BK21), Gyeongsang National University
  • Choong-Hoon Park Division of Applied Life Sciences (BK21), Gyeongsang National University,
  • Ki-Yong Kim National Institute of Animal Sciences, 77 Chuksan Gil, Suwon 441-706
  • Byung-Hyun Lee Division of Applied Life Sciences (BK21), Gyeongsang National University,

DOI:

https://doi.org/10.3329/ptcb.v17i2.3240

Keywords:

Dactylis golomerata, Orchardgrass, Mature seeds, Additives, Regeneration, Maltose

Abstract

A suitable callus induction and efficient regeneration protocol for orchardgrass (Dactylis golomerata L.) was developed. It consisted of 3 mg/l 2,4-D + 0.1 mg/l BA + 1 g/l CH + 300 mg/l L-proline + 40 mg/l L-cysteine + 30 g/l sucrose in MS showed the highest percentage of callus induction. Maltose exhibited better in regeneration than other types of carbon sources. Highest (71%) regeneration was obtained from N6 medium containing 1 mg/l 2,4-D + 3 mg/l BA + 1 g/l CH + 300 mg/l L-proline + 40 mg/l L-cysteine + 30 g/l maltose. Among the nine cultivars of orchardgrass (Dactylis golomerata L.), genotypic variation was observed in both callus induction and regeneration. Overall callus induction and regeneration rates were 23 - 73 and 17 - 71%, respectively.

 

Key words: Dactylis golomerata, Orchardgrass, Mature seeds, Additives, Regeneration, Maltose.

 

D.O.I. 10.3329/ptcb.v17i2.3240

 

Plant Tissue Cult. & Biotech. 17(2): 193-207, 2007 (December)

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How to Cite

Lee, S.-H., Ahsan, N., Lee, K.-W., Lee, D.-G., Alam, I., Kim, K.-H., Park, C.-H., Kim, K.-Y., & Lee, B.-H. (2009). Re-evaluation of Mature Seed-derived Callusing and Regeneration Potential of Nine Orchardgrass (Dactylis golomerata L.) Cultivars. Plant Tissue Culture and Biotechnology, 17(2), 193–207. https://doi.org/10.3329/ptcb.v17i2.3240

Issue

Vol. 17 No. 2 (2007)

Section

Articles