Agrobacterium-mediated Genetic Transformation of Two Varieties of Jute (Corchorus capsularis L.)

Authors

  • Rakha Hari Sarker Plant Breeding and Biotechnology Laboratory, Department of Botany, University of
  • G.M. Al-Amin Plant Breeding and Biotechnology Laboratory, Department of Botany, University of
  • Fathi Hassan Institute of Plant Genetics, Leibniz University of Hannover
  • M.I. Hoque Plant Breeding and Biotechnology Laboratory, Department of Botany, University of

DOI:

https://doi.org/10.3329/ptcb.v18i1.3245

Keywords:

Jute, Transformation, GUS expression, PCR analysis

Abstract

Transformation experiments were carried out using different explants of two varieties of white jute (Corchorus capsularis L.), namely, CVL-1 and CVE-3 with Agrobacterium tumefaciens strain (LBA4404/pBI121) containing the GUS and nptII genes. Maximum transformation ability was obtained from petiole-attached cotyledons and mature embryo explants. Kanamycin at a concentration of 200 mg/l was found optimum for selection of transformed shoots developed from

mature embryos. Histochemical assay revealed the stable expression of the GUS gene within the various tissues of transformed plantlets. Stable integration of GUS and nptII genes were confirmed by PCR analysis of genomic DNA isolated from these transformed shoots.

 

Key words: Jute, Transformation, GUS expression, PCR analysis

 

D.O.I. 10.3329/ptcb.v18i1.3245

 

Plant Tissue Cult. & Biotech. 18(1): 7-16, 2008 (June)

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How to Cite

Sarker, R. H., Al-Amin, G., Hassan, F., & Hoque, M. (2009). Agrobacterium-mediated Genetic Transformation of Two Varieties of Jute (Corchorus capsularis L.). Plant Tissue Culture and Biotechnology, 18(1), 7–16. https://doi.org/10.3329/ptcb.v18i1.3245

Issue

Vol. 18 No. 1 (2008)

Section

Articles