Aseptic Germination and Agrobacterium rhizogenesmediated Transformation of Taraxacum hybernum Steven

Authors

  • Aleksey Knyazev Institute of Biochemistry and Genetics, Ufa Scientific Centre, Russian Academy of Sciences, Ufa
  • Bulat Kuluev Institute of Biochemistry and Genetics, Ufa Scientific Centre, Russian Academy of Sciences, Ufa
  • Alexander Fateryga T.I. Vyazemsky Karadag Scientific Station ? Nature Reserve of RAS, Feodosiya
  • Gulnar Yasybaeva Institute of Biochemistry and Genetics, Ufa Scientific Centre, Russian Academy of Sciences, Ufa
  • Aleksey Chemeris Institute of Biochemistry and Genetics, Ufa Scientific Centre, Russian Academy of Sciences, Ufa

DOI:

https://doi.org/10.3329/ptcb.v27i2.35019

Keywords:

Taraxacum hybernum, Taraxacum kok‐saghyz, Russian Dandelion, Agrobacterium rhizogenes, Hairy Roots, Transgenic Plants, Natural Rubber

Abstract

Taraxacum hybernum Steven, also known as krym‐saghyz, is one of the potential sources of natural rubber. The authors studied the aseptic germination of the T. hybernum seeds using various sterilizing agents and their effect on contamination. In vitro T. hybernum plants were used for Agrobacterium rhizogenesmediated transformation. A4 and 15834 strains of A. rhizogenes showed similar effectiveness after inoculation by injection into the hypocotyls. Authors generated the hairy roots of T. hybernum and the transgenic forms of this plant using A. rhizogenes‐mediated transformation.

Plant Tissue Cult. & Biotech. 27(2): 141-151, 2017 (December)

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Published

2017-12-27

How to Cite

Knyazev, A., Kuluev, B., Fateryga, A., Yasybaeva, G., & Chemeris, A. (2017). Aseptic Germination and Agrobacterium rhizogenesmediated Transformation of Taraxacum hybernum Steven. Plant Tissue Culture and Biotechnology, 27(2), 141–151. https://doi.org/10.3329/ptcb.v27i2.35019

Issue

Vol. 27 No. 2 (2017)

Section

Articles